Mouse Anti-NHP PVR Recombinant Antibody (clone D171)
CAT#: VS-1024-XY79
This mouse-derived monoclonal antibody is designed to target PVR and is effective in detecting human, mouse, and non-human primate PVR. Generated using HeLa cells as the immunogen, it ensures precise recognition of the PVR protein. Suitable for Western blotting (WB), immunofluorescence (IF), and flow cytometry (FC) applications, this antibody provides exceptional versatility for studying PVR expression and localization. Its broad reactivity and reliable performance across multiple techniques make it a valuable asset for advanced research and analysis.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate cytoplasmic and membranous positivity in islets of Langerhans.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/29673_A_2_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/29673_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/29673_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/29673_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/29673_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/29673_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12568/29673_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073008-PVR/subcellular
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Specifications
- Immunogen
- HeLa cells
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PVR
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- D171
- Applications
- WB, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.2% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:10-1:100, IF: 1:10-1:100, FC: 2 µg/test
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone D171"
- CAT
- Product Name
See other products for "PVR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3400z | Mouse Anti-PVR Recombinant Antibody (clone 35G12) | WB, FC, ICC, IF | Mouse IgG1 |
| MOB-0791MZ | Recombinant Mouse Anti-Human PVR Antibody (clone E282) | FC, ICC, IF | Mouse antibody |
| FN-074CQ | Recombinant Mouse Anti-PVR Antibody (4.24) | FC, FuncS | Mouse IgG2a |
| VS3-FY1229 | Recombinant Rabbit Anti-PVR Antibody (clone R04-2C7) | WB, IHC-P | Rabbit IgG |
| VS3-FY2066 | Rabbit Anti-PVR Recombinant Antibody (clone R01-8A1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1899CQ | Recombinant Mouse Anti-PVR Antibody (D171) | BL, FC, ICC, IF | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2945 | Hi-Affi™ Recombinant Rabbit Anti-Pvr Monoclonal Antibody (DS2945AB) | ELISA, WB | IgG |
| MOR-4381 | Hi-Affi™ Recombinant Rabbit Anti-PVR Monoclonal Antibody (SI472DS) | WB, ELISA, IP | IgG |
| MOR-4382 | Hi-Affi™ Recombinant Rabbit Anti-PVR Monoclonal Antibody (SI473DS) | ICC, IF, IF, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1373 | AbPlus™ Anti-PVR Magnetic Beads (VS-0724-YC1373) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY188 | CytoStream™ Rat Anti-PVR Recombinant Antibody (clone TX56) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1833 | Anti-PVR Immunohistochemistry Kit | IHC | |
| VS-0525-XY5892 | Anti-Human PVR Immunohistochemistry Kit | IHC |
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