Mouse Anti-NHP SKP1 Recombinant Antibody (clone 4E11)
CAT#: VS-1024-XY482
Engineered for the detection of SKP1, this antibody demonstrates robust reactivity with human, mouse, and non-human primate samples. The immunogen is a purified recombinant fragment of human SKP1, ensuring high specificity and affinity. Suitable for an array of applications, including Western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, and ELISA, it provides versatile performance in diverse experimental settings. Researchers focused on cell cycle regulation and ubiquitin-mediated processes will find this antibody a critical resource for obtaining consistent and reliable results.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53745/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58134/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_6_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_B_7_5.jpg
Normal Tissue
Figure 6 Cerebellum
Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_B_7_8.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_8_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113558-SKP1
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Specifications
- Immunogen
- Purified recombinant fragment of human SKP1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SKP1
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 4E11
- Applications
- WB, IHC, ICC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ICC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
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Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 4E11"
- CAT
- Product Name
See other products for "SKP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3274 | Hi-Affi™ Recombinant Rabbit Anti-SKP1 Monoclonal Antibody (DS3274AB) | WB, FC, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1015 | Mouse Anti-SKP1 Recombinant Antibody (clone 4G3-G7-F6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK573 | Rabbit Anti-SKP1 Recombinant Antibody (clone JM27-37) | WB, ELISA | Rabbit IgG |
| VS3-XY1424 | Mouse Anti-SKP1 Recombinant Antibody (clone 4E11C10) | IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR866 | Mouse Anti-SKP1 Monoclonal Antibody (clone 1H9) | WB, IHC, FC | Mouse IgG1 |
| VS7-0425-WR867 | Mouse Anti-SKP1 Monoclonal Antibody (clone 4E11) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2079 | Anti-SKP1 Immunohistochemistry Kit | IHC |
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