Mouse Anti-NHP SKP1 Recombinant Antibody (clone 4E11) (CAT#: VS-1024-XY482)
Engineered for the detection of SKP1, this antibody demonstrates robust reactivity with human, mouse, and non-human primate samples. The immunogen is a purified recombinant fragment of human SKP1, ensuring high specificity and affinity. Suitable for an array of applications, including Western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, and ELISA, it provides versatile performance in diverse experimental settings. Researchers focused on cell cycle regulation and ubiquitin-mediated processes will find this antibody a critical resource for obtaining consistent and reliable results.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53745/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53745/if_selected.jpg
Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58134/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58134/if_selected.jpg
Figure 3 IHC staining of human gallbladder
(Immunohistochemical staining of human gallbladder shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_6_4_selected.jpg
(Immunohistochemical staining of human gallbladder shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_6_4_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_7_8.jpg
Figure 5 Cerebral cortex
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_B_7_5.jpg
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_B_7_5.jpg
Figure 6 Cerebellum
(Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_B_7_8.jpg
(Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_B_7_8.jpg
Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12982/31727_A_8_3.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113558-SKP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113558-SKP1
Specifications
- Immunogen
- Purified recombinant fragment of human SKP1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SKP1
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 4E11
- Applications
- WB, IHC, ICC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ICC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
- Alternative Names
- S-Phase Kinase Associated Protein 1; Transcription Elongation Factor B Polypeptide 1-Like; Cyclin-A/CDK2-Associated Protein P19; Organ Of Corti Protein II; Organ Of Corti Protein 2; P19skp1; OCP-II; TCEB1L; SKP1A; EMC19
- Gene ID
- 6500
- UniProt ID
- P63208
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1783MZ | Recombinant Mouse Anti-Human SKP1 Antibody | WB | Mouse antibody |
| VS3-QX1015 | Mouse Anti-SKP1 Recombinant Antibody (clone 4G3-G7-F6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK573 | Rabbit Anti-SKP1 Recombinant Antibody (clone JM27-37) | WB, ELISA | Rabbit IgG |
| VS3-XY1424 | Mouse Anti-SKP1 Recombinant Antibody (clone 4E11C10) | IHC, ICC, FC | Mouse IgG1 |
| VS3-FY1342 | Recombinant Rabbit Anti-SKP1 Antibody (clone R01-6H2) | WB, ICC, IF | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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