Mouse Anti-NHP USP9X Recombinant Antibody (clone OTI2G7)
CAT#: VS-1024-XY537
Designed for extensive utility, the USP9X antibody offers broad reactivity across species, including dog, human, mouse, non-human primate, and rat. Generated using a recombinant human protein fragment as the immunogen, this antibody ensures precise detection of USP9X in various applications, such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Its specificity and versatility make it an excellent choice for research involving multiple species and complex cellular processes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47417/1509_G5_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11618/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human bronchus
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70164/150726_B_6_2_selected.jpg
Normal Tissue
Figure 4 Parathyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11618/27556_A_3_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11618/27556_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11618/27556_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11618/27556_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124486-USP9X
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Specifications
- Immunogen
- Human recombinant protein fragment
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- USP9X
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI2G7
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein G purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, IF: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "USP9X"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2186CT | Recombinant Mouse anti-Human USP9X Monoclonal antibody (EML893) | WB | |
| MRO-1565-CN | Rabbit Anti-USP9X Recombinant Antibody (clone JG35-11) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3813 | Rabbit Anti-USP9X Recombinant Antibody (clone DS3813AB) | IHC-P, WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7792 | Anti-USP9X Immunohistochemistry Kit | IHC | |
| VS-0525-XY7793 | Anti-Mouse USP9X Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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