Mouse Anti-NPM1 Recombinant Antibody (clone ABT-NPM) (CAT#: VS3-CJ453)

• Immunogen
• Synthesized peptide derived from human Nucleophosmin

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human NPM1

• Species Reactivity
• Human

• Clone
• ABT-NPM

• Applications
• WB, IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• B23; NPM

• Gene ID
• 4869

• UniProt ID
• P06748

• Sequence Similarities
• Belongs to the nucleoplasmin family.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Nucleus

• Post Translation Modifications
• Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
  ADP-ribosylated.
  Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
  Sumoylated by ARF.
  Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36 (PubMed:19208757).

• Protein Refseq
• NP_001032827.1; NP_002511.1; NP_954654.1

• Function
• Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
  In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
  May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS