Mouse Anti-NR3C1 Monoclonal Antibody (VS7-0425-WR504) (CAT#: VS7-0425-WR504)
This product is a mouse IgG antibody that binds to human NR3C1. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
Figure 3 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
Figure 6 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
Specifications
- Immunogen
- Recombinant human NR3C1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NR3C1
- Species Reactivity
- Human
- Molecule Class
- Nuclear Receptor
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Glucocorticoid resistance, generalized (GCCR)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Immunohistochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- NR3C1; GCRST; Nuclear Receptor Subfamily 3 Group C Member 1; Glucocorticoid Nuclear Receptor Variant 1; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); GCCR; GCR
- Gene ID
- 2908
- UniProt ID
- P04150
- Long Name
- Nuclear Receptor Subfamily 3 Group C Member 1
- Cellular Localization
- Chromosome; Cytoplasm; Cytoskeleton; Mitochondrion; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000167.1
- Function
- Chromatin regulator; DNA-binding; Receptor; RNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0756MZ | Recombinant Mouse Anti-Human NR3C1 Antibody (clone 9F0) | ELISA | Mouse antibody |
| MRO-0664-CN | Recombinant Rabbit Anti-NR3C1 Monoclonal Antibody (JF0952) | WB, FC | Rabbit IgG |
| MRO-0665-CN | Recombinant Mouse Anti-NR3C1 Monoclonal Antibody (3F1) | WB, IF, IHC, FC | Mouse IgG2a |
| MRO-0666-CN | Recombinant Mouse Anti-NR3C1 Monoclonal Antibody (3F2) | WB, IHC, FC | Mouse IgG1 |
| VS3-XY1182 | Mouse Anti-NR3C1 Recombinant Antibody (clone 6E6) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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