Mouse Anti-NR3C1 Monoclonal Antibody (VS7-0425-WR504)
CAT#: VS7-0425-WR504
This product is a mouse IgG antibody that binds to human NR3C1. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
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Specifications
- Immunogen
- Recombinant human NR3C1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NR3C1
- Species Reactivity
- Human
- Molecule Class
- Nuclear Receptor
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Glucocorticoid resistance, generalized (GCCR)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Immunohistochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- NR3C1; GCRST; Nuclear Receptor Subfamily 3 Group C Member 1; Glucocorticoid Nuclear Receptor Variant 1; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); GCCR; GCR
- Gene ID
- 2908
- UniProt ID
- P04150
- Long Name
- Nuclear Receptor Subfamily 3 Group C Member 1
- Cellular Localization
- Chromosome; Cytoplasm; Cytoskeleton; Mitochondrion; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000167.1
- Function
- Chromatin regulator; DNA-binding; Receptor; RNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NR3C1"
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-2493 | Hi-Affi™ Recombinant Rabbit Anti-NR3C1 Monoclonal Antibody (DS2493AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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