Rabbit Anti-NR3C1 Recombinant Antibody (clone 2D8) (CAT#: ZG-0489U)
Rabbit Anti-NR3C1 Antibody (clone 2D8) displayed high specificity against the NR3C1 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-NR3C1 Antibody (ZG-0489U) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate
All lanes: NR3C1 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86, 83, 82, 77, 76, 65, 61, 52, 51, 50 kDa
Observed band size: 95 kDa
FC
Figure 2 Rabbit Anti-NR3C1 Antibody (ZG-0489U) in FC
Overlay histogram showing Jurkat cells stained with ZG-0489U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human GR
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NR3C1
- Species Reactivity
- Human
- Clone
- 2D8
- Applications
- ELISA, WB, FC
- Conjugate
- Unconjugated
- Related Disease
- Glucocorticoid Resistance, Generalized and Post-Traumatic Stress Disorder.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, FC:1:20-1:200
Target
- Alternative Names
- Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL
- Gene ID
- 2908
- UniProt ID
- P04150
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NR3C1"
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1788CQ | Recombinant Mouse Anti-NR3C1 Antibody (BuGR2) | WB, Neut, ChIP, FC, GS, ICC/IF, IHC, IHC-P, IP | IgG2 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2493 | Hi-Affi™ Recombinant Rabbit Anti-NR3C1 Monoclonal Antibody (DS2493AB) | WB, FC | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0664-CN | Recombinant Rabbit Anti-NR3C1 Monoclonal Antibody (JF0952) | WB, FC | Rabbit IgG |
| MRO-0665-CN | Recombinant Mouse Anti-NR3C1 Monoclonal Antibody (3F1) | WB, IF, IHC, FC | Mouse IgG2a |
| MRO-0666-CN | Recombinant Mouse Anti-NR3C1 Monoclonal Antibody (3F2) | WB, IHC, FC | Mouse IgG1 |
| VS3-WK398 | Rabbit Anti-NR3C1 Recombinant Antibody (clone JF0952) | WB, FC | Rabbit IgG |
| VS3-FY1067 | Recombinant Rabbit Anti-Nr3C1 Antibody (clone R04-8C8) | WB, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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