AbPlus™ Anti-NT5E Magnetic Beads (VS-0724-YC1493) (CAT#: VS-0724-YC1493)
The AbPlus Anti-NT5E Magnetic Beads (VS-0724-YC1493) is an innovative affinity resin which is bound with anti-NT5E specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of NT5E protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg

Figure 2 IHC staining of human endometrium
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg

Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg

Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg

Figure 7 Cervix
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg

Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg

Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-NT5E specific antibody
- Target
- NT5E
- Immunogen
- Recombinant human CD73/NT5E, aa 1-547.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
- Alternative Names
- 5'-Nucleotidase Ecto; 5-Nucleotidase Ecto; Ecto-5-Nucleotidase; EC 3.1.3.5; 5-NT; NT5; NTE; 5-Nucleotidase, Ecto (CD73); Purine 5-Prime-Nucleotidase; 5 Nucleotidase (CD73);
- Gene ID
- 4907
- UniProt ID
- P21589
Downloads
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1936z-S(P) | Recombinant Anti-Human NT5E Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
HPAB-M0172-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0172-YC-S(P)) | FC, Inhib | Mouse scFv |
HPAB-M0173-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0173-YC-S(P)) | FC, FuncS | Mouse scFv |
HPAB-N0196-YC-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-N0196-YC-S(P)) | FC | Human scFv |
HPAB-0500-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0500-FY-S(P)) | Neut, FC | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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