Hi-Affi™ Recombinant Rabbit Anti-NT5E Monoclonal Antibody (DS2503AB)
CAT#: MOR-2503
Recombinant Rabbit anti-NT5E monoclonal antibody for WB, IHC, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
Normal Tissue
Figure 2 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
Normal Tissue
Figure 7 Cervix
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
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Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS2503AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry; Immunocytochemistry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-593MZ | Human Anti-NT5E Recombinant Antibody (TAB-593MZ) | FC | Human IgG |
| TAB-594MZ | Human Anti-NT5E Recombinant Antibody (TAB-594MZ) | FC | Human IgG |
| TAB-595MZ | Human Anti-NT5E Recombinant Antibody (TAB-595MZ) | FC, FuncS | Human IgG |
| TAB-596MZ | Human Anti-NT5E Recombinant Antibody (TAB-596MZ) | FC | Human IgG |
| TAB-597MZ | Human Anti-NT5E Recombinant Antibody (TAB-597MZ) | FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1799CQ | Recombinant Mouse Anti-NT5E Antibody (NEUT-1799CQ) | FC, IHC-Fr, IF, IP, Inhib | IgG2a, κ |
| NEUT-1800CQ | Recombinant Rat Anti-Nt5e Antibody (TY/23) | BL | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0374-YC | Mouse Anti-NT5E Recombinant Antibody (HPAB-N0374-YC) | ELISA, FC, Inhib | Mouse IgG |
| HPAB-N0375-YC | Human Anti-NT5E Recombinant Antibody (HPAB-N0375-YC) | ELISA, FC, Inhib | Human IgG |
| HPAB-N0196-YC | Human Anti-NT5E Recombinant Antibody (HPAB-N0196-YC) | FC | Human IgG |
| HPAB-0500-FY | Mouse Anti-NT5E Recombinant Antibody (HPAB-0500-FY) | Neut, FC, Block, FuncS, WB | Mouse IgG |
| HPAB-0501-FY | Mouse Anti-NT5E Recombinant Antibody (HPAB-0501-FY) | Neut, FC, Block, FuncS, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0374-YC-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0374-YC-F(E)) | ELISA, FC, Inhib | Mouse Fab |
| HPAB-N0375-YC-F(E) | Human Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-N0375-YC-F(E)) | ELISA, FC, Inhib | Human Fab |
| HPAB-0500-FY-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0500-FY-F(E)) | Neut, FC | Mouse Fab |
| HPAB-0501-FY-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0501-FY-F(E)) | Neut, FC | Mouse Fab |
| HPAB-0502-FY-F(E) | Mouse Anti-NT5E Recombinant Antibody; Fab Fragment (HPAB-0502-FY-F(E)) | Neut, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-441CQ | Afuco™ Anti-NT5E ADCC Recombinant Antibody, ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0172-YC-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-M0172-YC-S(P)) | FC, Inhib | Mouse scFv |
| HPAB-0500-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0500-FY-S(P)) | Neut, FC | Mouse scFv |
| HPAB-0501-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0501-FY-S(P)) | Neut, FC | Mouse scFv |
| HPAB-0502-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0502-FY-S(P)) | Neut, FC | Mouse scFv |
| HPAB-0503-FY-S(P) | Mouse Anti-NT5E Recombinant Antibody; scFv Fragment (HPAB-0503-FY-S(P)) | Neut, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1442 | AbPlus™ Anti-Nt5e Magnetic Beads (VS-0724-YC1442) | IP, Protein Purification | |
| VS-0724-YC1493 | AbPlus™ Anti-NT5E Magnetic Beads (VS-0724-YC1493) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC844 | CytoStream™ Mouse Anti-NT5E Recombinant Antibody (VS13-YC844) | WB, IHC-P, ICC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY59 | Human Anti-NT5E (clone Uliledlimab) scFv-Fc Chimera | FC, ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC110 | Recombinant Anti-NT5E Vesicular Antibody, EV Displayed (VS-0425-YC110) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5002 | Anti-NT5E Immunohistochemistry Kit | IHC | |
| VS-0525-XY5003 | Anti-Mouse NT5E Immunohistochemistry Kit | IHC | |
| VS-0525-XY5004 | Anti-Rat NT5E Immunohistochemistry Kit | IHC |
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