CytoStream™ Mouse Anti-NT5E Recombinant Antibody (VS13-YC844) (CAT#: VS13-YC844)
The CytoStream™ Mouse Anti-NT5E Recombinant Antibody is specific for human NT5E. NT5E encodes a membrane protein converting nucleotides to nucleosides, implicated in calcification disorders, with splice variants encoding different isoforms. The anti-NT5E antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/1781_H2_30_cr596dceb1b7bc9_selected.jpg
Figure 2 IHC staining of human endometrium
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
(Immunohistochemical staining of human endometrium shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_2_3_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_5_6.jpg
Figure 7 Cervix
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_B_7_3.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17357/41086_A_7_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135318-NT5E
Specifications
- Immunogen
- Recombinant human CD73, aa 27-549.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NT5E
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- Cardiovascular Disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunohistochemistry-paraffin (IHC-P): 1/200-1/600
Immunofluorescence (IF): 1/100
Flow cytometry (FC): 1/100
Target
- Alternative Names
- 5'-Nucleotidase Ecto; 5-Nucleotidase Ecto; Ecto-5-Nucleotidase; EC 3.1.3.5; 5-NT; NT5; NTE; 5-Nucleotidase, Ecto (CD73); Purine 5-Prime-Nucleotidase; 5 Nucleotidase (CD73);
- Gene ID
- 4907
- UniProt ID
- P21589
- Long Name
- 5'-Nucleotidase Ecto
- Cellular Localization
- Cell membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-593MZ-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (TAB-593MZ-S(P)) | FC | Human scFv |
| TAB-594MZ-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (TAB-594MZ-S(P)) | FC | Human scFv |
| TAB-595MZ-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (TAB-595MZ-S(P)) | FC | Human scFv |
| TAB-596MZ-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (TAB-596MZ-S(P)) | FC | Human scFv |
| TAB-597MZ-S(P) | Human Anti-NT5E Recombinant Antibody; scFv Fragment (TAB-597MZ-S(P)) | FC | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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