Rabbit Anti-NTN1 Recombinant Antibody (VS13-YC845)
CAT#: VS13-YC845
The Rabbit Anti-NTN1 Recombinant Antibody is specific for human, mouse and rat NTN1. NTN1 encodes netrin, thought to guide axons and mediate cell migration, with mutations and expression loss linked to cancer development. The anti-NTN1 antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56419/912_A3_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56419/132808_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56419/132808_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56419/132808_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Medium Intensity: Strong Quantity: <25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56419/132808_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO, T-47d, U-2 OS, U-251 MG, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065320-NTN1
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Specifications
- Immunogen
- Recombinant human Netrin 1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NTN1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NTN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-230CQ | Mouse Anti-NTN1 Recombinant Antibody (TAB-230CQ) | ELISA, FuncS, Inhib | Mouse IgG1 |
| TAB-230CQ-S(P) | Mouse Anti-NTN1 Recombinant Antibody; scFv Fragment (TAB-230CQ-S(P)) | ELISA, FC, WB | Mouse scFv |
| TAB-230CQ-F(E) | Mouse Anti-NTN1 Recombinant Antibody; Fab Fragment (TAB-230CQ-F(E)) | ELISA, FC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-231CQ | Human Anti-NTN1 Recombinant Antibody (TAB-231CQ) | ELISA, FC, WB | Human IgG1 |
| TAB-231CQ-S(P) | Human Anti-NTN1 Recombinant Antibody; scFv Fragment (TAB-231CQ-S(P)) | ELISA, FC, WB | Human scFv |
| TAB-231CQ-F(E) | Human Anti-NTN1 Recombinant Antibody; Fab Fragment (TAB-231CQ-F(E)) | ELISA, FC, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1427MZ | Recombinant Mouse Anti-Mouse NTN1 Antibody (clone 269047) | ELISA, WB | Mouse antibody |
| FN-017CQ | Recombinant Human Anti-NTN1 Antibody (FN-017CQ) | ELISA, FuncS | Human IgG2, λ |
| FN-051CQ | Recombinant Human Anti-NTN1 Antibody (FN-051CQ) | ELISA, FuncS | Human IgG2, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0723MZ | Chicken Anti-NTN1 Polyclonal IgY | ICC, IF, IHC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2505 | Hi-Affi™ Recombinant Rabbit Anti-NTN1 Monoclonal Antibody (DS2505AB) | WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5012 | Anti-NTN1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC242 | Recombinant Anti-NTN1 Eliminating Antibody, pH-Sensitive (VS-0625-YC242) | Antigen-Sweeping In Vivo. | Mouse IgG1 |
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