Rabbit Anti-OGT Recombinant Antibody (clone 8G7) (CAT#: ZG-0492U)
Rabbit Anti-OGT Antibody (clone 8G7) displayed high specificity against the OGT protein of Human, Mouse and Rat.
Specific Inquiry
WB
Figure 1 Rabbit Anti-OGT Antibody (ZG-0492U) in WB
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, HL-60 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate
All lanes: OGT antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 117, 104, 116, 75 kDa
Observed band size: 117 kDa
IHC
Figure 2 Rabbit Anti-OGT Antibody (ZG-0492U) in IHC
IHC image of ZG-0492U diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human OGT / O-Linked N-Acetylglucosamine Transferase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human OGT
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 8G7
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Intellectual Developmental Disorder, X-Linked 106 and Myoepithelioma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; EC 2.4.1.255; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT; OGT
- Gene ID
- 8473
- UniProt ID
- O15294
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1129-CN | Recombinant Rabbit Anti-OGT Monoclonal Antibody (JB44-39) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK308 | Rabbit Anti-OGT Recombinant Antibody (clone JB44-39) | WB, ELISA | Rabbit IgG |
| VS3-FY1080 | Recombinant Rabbit Anti-OGT Antibody (clone R05-5C2) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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