Recombinant Rabbit Anti-OGT Antibody (clone R05-5C2)
CAT#: VS3-FY1080
This product is a recombinant rabbit antibody that recognizes OGT. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-5C2 is specific for human, mouse, rat OGT. The antigen is synthetic peptide of human ogt/o-linked n-acetylglucosamine transferase.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30753/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34099/ihc_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30751/68057_A_4_6.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30751/68057_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30751/68057_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147162-OGT
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Specifications
- Immunogen
- Synthetic peptide of human OGT/O-linked N-acetylglucosamine transferase.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat OGT
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-5C2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 117 kDa; Observed MW: 117 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- O-Linked N-Acetylglucosamine (GlcNAc) Transferase; O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:Polypeptide-N-Acetylglucosaminyl Transferase); UDP-N-Acetylglucosamine:Polypeptide-N-Acetylglucosaminyl Transferase; O-Linked N-Acetylglucosamine Transferase 110 KDa Subunit; O-GlcNAc Transferase Subunit P110; Uridinediphospho-N-Acetylglucosamine:Polypeptide Beta-N-Acetylglucosaminyl Transferase; UDP-N-Acetylglucosamine--Peptide N-Acetylglucosaminyltransferase 110 KDa Subunit; O-GlcNAc Transferase P110 Subunit; EC 2.4.1.186
- Gene ID
- 8473
- UniProt ID
- O15294
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "OGT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1129-CN | Recombinant Rabbit Anti-OGT Monoclonal Antibody (JB44-39) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0492U | Rabbit Anti-OGT Recombinant Antibody (clone 8G7) | ELISA, WB, IHC | Rabbit IgG |
| VS3-WK308 | Rabbit Anti-OGT Recombinant Antibody (clone JB44-39) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC329 | AbPlus™ Anti-OGT Magnetic Beads (VS-0724-YC329) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC856 | CytoStream™ Rabbit Anti-OGT Recombinant Antibody (VS13-YC856) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1540 | Anti-OGT Immunohistochemistry Kit | IHC | |
| VS-0525-XY5082 | Anti-Mouse OGT Immunohistochemistry Kit | IHC | |
| VS-0525-XY5081 | Anti-Human OGT Immunohistochemistry Kit | IHC |
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