Mouse Anti-P2RX7 Recombinant Antibody (clone 7G1D6)
CAT#: VS7-0425-WR535
This product is a mouse IgG2b antibody that binds to human P2RX7. The antibody 7G1D6 recognizes specific amino acid domain of human P2RX7 (AA: 226-452). It can be used for WB, IHC, and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34968/68670_B_8_5_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows weak positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42013/103582_B_7_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34968/68670_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34968/68670_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34968/68670_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34968/68670_A_4_6.jpg
Normal Tissue
Figure 7 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34968/68670_B_8_1.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707, RPMI-8226, SK-MEL-30, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089041-P2RX7
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Specifications
- Immunogen
- Recombinant human P2RX7 (AA: 226-452) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human P2RX7
- Species Reactivity
- Human
- Clone
- 7G1D6
- Molecule Class
- P2X Receptor Family
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- P2RX7; P2X7; ATP Receptor; Purinergic Receptor P2X7 Variant A; Purinergic Receptor; P2Z Receptor; P2X Purinoceptor 7; Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7
- Gene ID
- 5027
- UniProt ID
- Q99572
- Long Name
- Purinergic Receptor P2X 7
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- ADP-ribosylation; Disulfide bond; Glycoprotein; Lipoprotein; Palmitate; Phosphoprotein
- Protein Refseq
- NP_002553.3
- Function
- Ion channel; Ligand-gated ion channel; Receptor
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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