Rabbit Anti-PARP1 Recombinant Antibody (clone 7C11) (CAT#: ZG-0499U)
Rabbit Anti-PARP1 Antibody (clone 7C11) displayed high specificity against the PARP1 protein of Human.
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Custom Production
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WB
Figure 1 Rabbit Anti-PARP1 Antibody (ZG-0499U) in WB
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: PARP antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
IHC
Figure 2 Rabbit Anti-PARP1 Antibody (ZG-0499U) in IHC
IHC image of ZG-0499U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-PARP1 Antibody (ZG-0499U) in IHC
IHC image of ZG-0499U diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PARP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PARP1
- Species Reactivity
- Human
- Clone
- 7C11
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Diphtheria and Xeroderma Pigmentosum, Complementation Group A.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Poly [ADP-ribose] polymerase 1; PARP-1; EC 2.4.2.30; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+ ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1; ADPRT PPOL
- Gene ID
- 142
- UniProt ID
- P09874
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2594 | Hi-Affi™ Recombinant Rabbit Anti-PARP1 Monoclonal Antibody (DS2594AB) | FC, ICC, IHC-P, IP, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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