Rabbit Anti-PBRM1 Recombinant Antibody (clone R04-4L8) (CAT#: VS3-XY3458)
It is a recombinant rabbit antibody that recognizes PBRM1 (Polybromo 1), which acts as a negative regulator of cell proliferation. PBRM1 involves in transcriptional activation and repression of select genes by chromatin remodeling. The antibody specifically reacts with human, mouse and rat PBRM1. This antibody has been used in Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry and Immunofluorescence.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/1395_H9_1_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/1395_H9_1_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34465_B_7_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34465_B_7_5.jpg
Figure 4 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34414_A_8_3.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34414_A_8_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34414_A_4_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34414_A_4_6.jpg
Figure 6 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34414_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15629/34414_A_9_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163939-PBRM1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163939-PBRM1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PBRM1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4L8
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Renal Cell Carcinoma; Nonpapillary; Clear Cell Papillary Renal Cell Carcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- PB1; RCC; BAF180; SMARCH1
- Gene ID
- 55193
- UniProt ID
- Q86U86
- Long Name
- Polybromo 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys754, Lys1398, Lys1626, and Lys1642 (NX_Q86U86 [NX_Q86U86-1])
Modification sites at PhosphoSitePlus (Q86U86)
Glycosylation from GlyGen (Q86U86) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_011532204.1
- Function
- Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).
Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF).
Acts as a negative regulator of cell proliferation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-430 | Recombinant Human Anti-Human PBRM1 Antibody, clone D466 | ELISA, IP-MS | IgG |
| VS3-WK1049 | Mouse Anti-PBRM1 Recombinant Antibody (VS3-WK1049) | WB | Mouse IgG |
| VS3-FY2830 | Mouse Anti-PBRM1 Recombinant Antibody (VS3-FY2830) | WB | Mouse IgG |
| VS13-YC873 | Rabbit Anti-PBRM1 Recombinant Antibody (VS13-YC873) | IHC-P | Rabbit IgG |
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