CytoStream™ Rabbit Anti-PDCD4 Recombinant Antibody (VS13-YC880) (CAT#: VS13-YC880)
The CytoStream™ Rabbit Anti-PDCD4 Recombinant Antibody is specific for human and mouse PDCD4. PDCD4 encodes a tumor suppressor inhibiting translation initiation, with alternative splicing producing multiple transcript variants. The anti-PDCD4 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27214/231_F5_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27214/231_F5_2_selected.jpg
Figure 2 Pancreas
(High expression in pancreas)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_2_3_val_selected.jpg
(High expression in pancreas)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_2_3_val_selected.jpg
Figure 3 IHC staining of human thyroid gland
(Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37024/78562_B_3_5_selected.jpg
(Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37024/78562_B_3_5_selected.jpg
Figure 4 Testis
(Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: Medium Intensity: Strong Quantity: <25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: Medium Intensity: Strong Quantity: <25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_6_6.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_7_8.jpg
(Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_7_8.jpg
Figure 6 Cerebral cortex
(Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_B_8_5.jpg
(Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_B_8_5.jpg
Figure 7 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_7_3.jpg
Figure 8 Kidney
(Bowman's capsule Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_7_5.jpg
(Bowman's capsule Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1032/2348_A_7_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (T-47d, U-266/70)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000150593-PDCD4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000150593-PDCD4
Specifications
- Immunogen
- Recombinant human PDCD4, aa 425-469.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PDCD4
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/20-1/50
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Programmed Cell Death 4; Programmed Cell Death 4 (Neoplastic Transformation Inhibitor); Neoplastic Transformation Inhibitor Protein; Nuclear Antigen H731; Protein 197/15a; H731; Programmed Cell Death Protein 4; Nuclear Antigen H731-Like;
- Gene ID
- 27250
- UniProt ID
- Q53EL6
- Long Name
- Programmed Cell Death 4
- Cellular Localization
- Nucleus Cytoplasm
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2839z | Mouse Anti-PDCD4 Recombinant Antibody (clone 42C2) | WB, ELISA | Mouse IgG1, κ |
| MOB-1301CT | Recombinant Mouse anti-Human PDCD4 Monoclonal antibody (L5D2) | ELISA, IHC-P, WB | |
| MRO-1192-CN | Recombinant Rabbit Anti-PDCD4 Monoclonal Antibody (JA80-83) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK268 | Rabbit Anti-PDCD4 Recombinant Antibody (clone JA80-83) | WB, ELISA | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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