Rabbit Anti-PDLIM1 Recombinant Antibody (clone R01-7A8)
CAT#: VS3-FY1821
This R01-7A8 recombinant monoclonal antibody exhibits specific binding to human PDLIM1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This PDLIM1 antibody was verified by western blot analysis of PDLIM1 in K562, C6, 3T3, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72840/1348_F5_4_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong positivity in islets of Langerhans.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_2_3_selected.jpg
Normal Tissue
Figure 4 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/153191_A_6_2_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_7_8.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_9_3.jpg
Normal Tissue
Figure 7 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_6_6.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HaCaT, Hep G2, HHSteC)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107438-PDLIM1
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WB
Figure 1 Anti-PDLIM1 Antibody (VS3-FY1821) in WB.
The PDLIM1 expression in K562, C6, 3T3, Hela lysates were detected using the anti-PDLIM1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PDLIM1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDLIM1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-7A8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
- Related Disease
- Supravalvular Aortic Stenosis and Williams-Beuren Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PDZ And LIM Domain 1; Elfin; Carboxyl Terminal LIM Domain Protein 1; LIM Domain Protein CLP-36; CLIM1; CLP36; Epididymis Secretory Protein Li 112
- Gene ID
- 9124
- UniProt ID
- O00151
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Ubiquitination at Lys22 (NX_O00151 [NX_O00151-1])
Modification sites at PhosphoSitePlus (O00151)
Glycosylation from GlyGen (O00151) 10 sites, 2 O-linked glycans (9 sites)
- Protein Refseq
- NP_066272.1
- Function
- Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PDLIM1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2647 | Hi-Affi™ Recombinant Rabbit Anti-PDLIM1 Monoclonal Antibody (DS2647AB) | WB, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1018 | AbPlus™ Anti-PDLIM1 Magnetic Beads (VS-0724-YC1018) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC890 | Rabbit Anti-PDLIM1 Recombinant Antibody (VS13-YC890) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5293 | Anti-PDLIM1 Immunohistochemistry Kit | IHC |
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