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PDLIM1
Rabbit Anti-PDLIM1 Recombinant Antibody (clone R01-7A8)

Rabbit Anti-PDLIM1 Recombinant Antibody (clone R01-7A8) (CAT#: VS3-FY1821)

Name: Rabbit Anti-PDLIM1 Recombinant Antibody (clone R01-7A8)
SKU: VS3-FY1821
Rating: 5 (1 reviews)

This R01-7A8 recombinant monoclonal antibody exhibits specific binding to human PDLIM1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This PDLIM1 antibody was verified by western blot analysis of PDLIM1 in K562, C6, 3T3, Hela lysates.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Anti-PDLIM1 Antibody (VS3-FY1821) in WB.

Figure 1 Anti-PDLIM1 Antibody (VS3-FY1821) in WB.

The PDLIM1 expression in K562, C6, 3T3, Hela lysates were detected using the anti-PDLIM1 by western blot assay.

Subcellular Location

Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/if_selected.jpg

Figure 2 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72840/1348_F5_4_selected.jpg

Normal Tissue

Figure 3 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong positivity in islets of Langerhans.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_2_3_selected.jpg

Figure 4 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/153191_A_6_2_selected.jpg

Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_7_8.jpg

Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_9_3.jpg

Figure 7 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_6_6.jpg

Figure 8 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_B_4_6.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enhanced (HaCaT, Hep G2, HHSteC)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107438-PDLIM1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human PDLIM1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat PDLIM1

Species Reactivity
Human, Mouse, Rat

Clone
R01-7A8

Applications
Western Blot, Immunofluorescence, Immunoprecipitation

MW
The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.

Related Disease
Supravalvular Aortic Stenosis and Williams-Beuren Syndrome

Product Property

Purification
Affinity Purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
PDZ And LIM Domain 1; Elfin; Carboxyl Terminal LIM Domain Protein 1; LIM Domain Protein CLP-36; CLIM1; CLP36; Epididymis Secretory Protein Li 112

Gene ID
9124

UniProt ID
O00151

Cellular Localization
Cytoplasm, Cytoskeleton

Post Translation Modifications
Ubiquitination at Lys22 (NX_O00151 [NX_O00151-1])
Modification sites at PhosphoSitePlus (O00151)
Glycosylation from GlyGen (O00151) 10 sites, 2 O-linked glycans (9 sites)

Protein Refseq
NP_066272.1

Function
Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts.

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody Products Antibody Magnetic Beads

CAT	Product Name	Application	Type
MOR-2647	Hi-Affi™ Recombinant Rabbit Anti-PDLIM1 Monoclonal Antibody (DS2647AB)	WB, ICC, FC	IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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