Rabbit Anti-PDLIM1 Recombinant Antibody (VS13-YC890) (CAT#: VS13-YC890)
The Rabbit Anti-PDLIM1 Recombinant Antibody is specific for human, mouse and rat PDLIM1. PDLIM1 encodes a cytoplasmic protein linked to the cytoskeleton, functioning as an adaptor for LIM-interacting proteins, with associated pseudogenes on various chromosomes. The anti-PDLIM1 antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/20).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/if_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/if_selected.jpg
Figure 2 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72840/1348_F5_4_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72840/1348_F5_4_selected.jpg
Figure 3 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong positivity in islets of Langerhans.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_2_3_selected.jpg
(Immunohistochemical staining of human pancreas shows strong positivity in islets of Langerhans.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_2_3_selected.jpg
Figure 4 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/153191_A_6_2_selected.jpg
(Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72838/153191_A_6_2_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_7_8.jpg
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_9_3.jpg
Figure 7 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_6_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_A_6_6.jpg
Figure 8 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_B_4_6.jpg
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17010/39298_B_4_6.jpg
Figure 9 RNA cell line category: Cell line enhanced (HaCaT, Hep G2, HHSteC)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107438-PDLIM1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107438-PDLIM1
Specifications
- Immunogen
- Recombinant human PDLIM1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDLIM1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- NONE
- Related Disease
- Supravalvular Aortic Stenosis and Williams-Beuren Syndrome
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- PDZ And LIM Domain 1; Elfin; Carboxyl Terminal LIM Domain Protein 1; LIM Domain Protein CLP-36; CLIM1; CLP36; Epididymis Secretory Protein Li 112;
- Gene ID
- 9124
- UniProt ID
- O00151
- Long Name
- PDZ And LIM Domain 1
- Cellular Localization
- Cytoplasm; Cytoskeleton
- Protein Refseq
- NP_066272.1
- Function
- Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2647 | Hi-Affi™ Recombinant Rabbit Anti-PDLIM1 Monoclonal Antibody (DS2647AB) | WB, ICC, FC | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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