Mouse Anti-PDPN Recombinant Antibody (clone D2-40) (CAT#: HPAB-0087-YJ)
This product is a mouse antibody that recognizes human MA2 (gp36, podoplanin, Aggrus, T1A-2). Podoplanin (PDPN) is an O-glycosylated transmembrane glycoprotein that is selectively expressed by, and is a marker of, lymphatic endothelial cells. The antibody D2-40 has been shown to be a sensitive and specific antibody for the detection of lymphatic endothelium in different malignancies, and functions in the routine evaluation of lymphatic invasion in esophageal cancer. Mab D2-40 was reported to be an excellent immunohistochemical marker of cutaneous Kaposi's sarcomas and may play a role in the differential diagnosis of epithelioid malignant mesothelioma versus adenocarcinoma.
Specific Inquiry
IHC
Figure 1 Histopathology and immunohistochemistry
(A, D) hematoxylin and eosin (H-E) stain, (B, C, E) immunoperoxidase stain for PDPN, hematoxylin counterstain, (A, B) × 50, (C) × 100; (D, E) × 75; scale bars, 100 μm.
Tsuneki, M., Yamazaki, M., Maruyama, S., Cheng, J., & Saku, T. (2013). Podoplanin-mediated cell adhesion through extracellular matrix in oral squamous cell carcinoma. Laboratory Investigation, 93(8), 921.
IF
Figure 2 Immunofluorescence
(left) PDPN, (right) preimmune IgG, Hoechest-33258 (blue) counterstain, × 100, scale bar, 100 μm.
Tsuneki, M., Yamazaki, M., Maruyama, S., Cheng, J., & Saku, T. (2013). Podoplanin-mediated cell adhesion through extracellular matrix in oral squamous cell carcinoma. Laboratory Investigation, 93(8), 921.
WB
Figure 3 Western blot analysis for PDPN and β-actin in cell lysates of SCC and ACC cells.
Tsuneki, M., Yamazaki, M., Maruyama, S., Cheng, J., & Saku, T. (2013). Podoplanin-mediated cell adhesion through extracellular matrix in oral squamous cell carcinoma. Laboratory Investigation, 93(8), 921.
Specifications
- Immunogen
- Resected tissue from dysgeminoma of the ovary
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PDPN
- Species Reactivity
- Human
- Clone
- D2-40
- Applications
- IHC, IF, WB
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody D2-40 has been reported in applications of Immunohistochemistry, Immunofluorescence, Western Blot. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Podoplanin; Lung Type I Cell Membrane Associated Glycoprotein; Glycoprotein 36; PA2.26 Antigen; T1-Alpha; AGGRUS; GP36; T1A; Lung Type-I Cell Membrane-Associated Glycoprotein (T1A-2); Glycoprotein, 36-KD; HT1alpha-1; M2A; D2-40
- Gene ID
- 10630
- UniProt ID
- Q86YL7
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PDPN"
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-650 | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (PSBL-650) | WB, FuncS | Mouse scFv |
| HPAB-0502-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0502-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
| HPAB-0503-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0503-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
| HPAB-0504-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0504-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
| HPAB-0505-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0505-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1055CL | Rat Anti-PDPN Recombinant Antibody (TAB-1055CL) | WB, FC | Rat IgG |
| TAB-1055CL-S(P) | Rat Anti-PDPN Recombinant Antibody; scFv Fragment (TAB-1055CL-S(P)) | SDS-PAGE, WB, FC | Rat scFv |
| TAB-1055CL-F(E) | Rat Anti-PDPN Recombinant Antibody; Fab Fragment (TAB-1055CL-F(E)) | SDS-PAGE, WB, FC | Rat Fab |
| TAB-0567CL-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (TAB-0567CL-S(P)) | WB, FC | Mouse scFv |
| TAB-0568CL-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (TAB-0568CL-S(P)) | WB, FC | Mouse scFv |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1851CQ | Recombinant Hamster Anti-Pdpn Antibody (8.1.1) | BL, IF, WB, FC | IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2650 | Hi-Affi™ Recombinant Rabbit Anti-PDPN Monoclonal Antibody (DS2650AB) | FC, WB | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0502-CN | Mouse Anti-PDPN Recombinant Antibody (HPAB-0502-CN) | WB, ELISA, FC, IP | Mouse IgG1, κ |
| HPAB-0503-CN | Human Anti-PDPN Recombinant Antibody (HPAB-0503-CN) | WB, ELISA, FC, IP | Chimeric (mouse/human) IgG1 |
| HPAB-0504-CN | Mouse Anti-PDPN Recombinant Antibody (HPAB-0504-CN) | WB, ELISA, FC, IP | Mouse IgG |
| HPAB-0505-CN | Human Anti-PDPN Recombinant Antibody (HPAB-0505-CN) | WB, ELISA, FC, IP | Chimeric (mouse/human) IgG1 |
| HPAB-0417-YC | Rat Anti-PDPN Recombinant Antibody (HPAB-0417-YC) | WB, FC, IHC, IP, Cyt | Rat IgG2a, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0502-CN-F(E) | Mouse Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0502-CN-F(E)) | WB, ELISA, FC, IP | Mouse Fab |
| HPAB-0503-CN-F(E) | Human Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0503-CN-F(E)) | WB, ELISA, FC, IP | Human Fab |
| HPAB-0504-CN-F(E) | Mouse Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0504-CN-F(E)) | WB, ELISA, FC, IP | Mouse Fab |
| HPAB-0505-CN-F(E) | Human Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0505-CN-F(E)) | WB, ELISA, FC, IP | Human Fab |
| HPAB-0417-YC-F(E) | Rat Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0417-YC-F(E)) | WB, FC, IHC, IP, Cyt | Rat Fab |
Therapeutic Protein
| CAT | Product Name | Application | Type |
|---|---|---|---|
| CBP-215YJL | Recombinant Human Podoplanin Protein | Positive Control, Immunogen, SDS-PAGE, WB, ELISA |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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