Mouse Anti-PDPN Recombinant Antibody (clone PMab-2) (CAT#: FAMAB-1296CQ)

Figure 1 Western blot analysis by PMab-2.

Total cell lysate were electrophoresed on 5-20% polyacrylamide gels and transferred onto a PVDF membrane. After blocking, the membrane was incubated with 0.1 mg/mL of PMab-2, and then with peroxidase-conjugated secondary antibodies; the membrane was detected using a Sayaca-Imager.

Oki, H., Honma, R., Ogasawara, S., Fujii, Y., Liu, X., Takagi, M., ... & Kato, Y. (2015). Development of sensitive monoclonal antibody PMab-2 against rat podoplanin. Monoclonal antibodies in immunodiagnosis and immunotherapy, 34(6), 396-403.

Figure 2 Flow cytometry.

Cells were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA. After washing with PBS, cells were treated with PMab-2, followed by treatment with secondary antibodies. Fluorescence data were collected using a Cell Analyzer EC800.

Oki, H., Honma, R., Ogasawara, S., Fujii, Y., Liu, X., Takagi, M., ... & Kato, Y. (2015). Development of sensitive monoclonal antibody PMab-2 against rat podoplanin. Monoclonal antibodies in immunodiagnosis and immunotherapy, 34(6), 396-403.

Figure 3 Immunohistochemical analysis by PMab-2.

Sections of rat lung were incubated with 0.5 mg/ml of PMab-2, followed by Envision+ kit, and color was developed using DAB and counterstained with hematoxylin. Scale bar, 100 μm.

Oki, H., Honma, R., Ogasawara, S., Fujii, Y., Liu, X., Takagi, M., ... & Kato, Y. (2015). Development of sensitive monoclonal antibody PMab-2 against rat podoplanin. Monoclonal antibodies in immunodiagnosis and immunotherapy, 34(6), 396-403.

Figure 4 Immunohistochemical analysis by PMab-2 in a dose-dependent manner.

Sections of rat kidney were incubated with 0.001-0.1 mg/mL of PMab-2, followed by Envision+ kit, and color was developed using DAB and counterstained with hematoxylin. Arrowheads, glomerulus. Scale bar, 100 μm.

Oki, H., Honma, R., Ogasawara, S., Fujii, Y., Liu, X., Takagi, M., ... & Kato, Y. (2015). Development of sensitive monoclonal antibody PMab-2 against rat podoplanin. Monoclonal antibodies in immunodiagnosis and immunotherapy, 34(6), 396-403.