Rabbit Anti-Phospho-MYB (S11) Recombinant Antibody (clone 3F4)
CAT#: ZG-0477U
Rabbit Anti-Phospho-MYB (S11) Antibody (clone 3F4) displayed high specificity against the MYB protein of Human.
Tested Data
Gene Expression
IF
Figure 1 Rabbit Anti-Phospho-MYB (S11) Antibody (ZG-0477U) in IF
Immunofluorescence staining of Hela cells with ZG-0477U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71605/1817_A4_6_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_A_5_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_A_6_6.jpg
Normal Tissue
Figure 8 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_B_6_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17704/41000_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HL-60, MOLT-4, NB-4, REH, THP-1, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118513-MYB
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-MYB (S11)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-MYB (S11)
- Species Reactivity
- Human
- Clone
- 3F4
- Applications
- ELISA, IF
- Conjugate
- Unconjugated
- Related Disease
- Angiocentric Glioma and Acute Basophilic Leukemia.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence. Specific conditions should be optimized by the end user.
IF:1:20-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MYB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2314z | Mouse Anti-MYB Recombinant Antibody (clone 40A5) | WB, FC, ICC, IF, IP | Mouse IgG1 |
| MOB-2193CT | Recombinant Mouse anti-Xenopus laevis myb Monoclonal antibody (6F22) | IHC-Fr, IP, WB | |
| MRO-2392-CN | Recombinant Rabbit Anti-MYB (phosphorylated Ser11) Monoclonal Antibody (CBACN-677) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ555 | Mouse Anti-MYB Recombinant Antibody (VS3-CJ555) | WB | Mouse IgG1, κ |
| VS3-CJ556 | Rabbit Anti-MYB (phospho S11) Recombinant Antibody (VS3-CJ556) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2332 | Hi-Affi™ Recombinant Rabbit Anti-MYB Monoclonal Antibody (DS2332AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4604 | Anti-MYB Immunohistochemistry Kit | IHC |
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