Rabbit Anti-Phospho-PRKCA (T638) Recombinant Antibody (clone 3A5) (CAT#: ZG-0522U)
Rabbit Anti-Phospho-PRKCA (T638) Antibody (clone 3A5) displayed high specificity against the PRKCA protein of Human.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-Phospho-PRKCA (T638) Antibody (ZG-0522U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-PRKCA antibody at 0.68μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 80 KDa
Observed band size: 80 KDa
IF
Figure 2 Rabbit Anti-Phospho-PRKCA (T638) Antibody (ZG-0522U) in IF
Immunofluorescence staining of HepG2 cells with ZG-0522U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-PRKCA (T638)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-PRKCA (T638)
- Species Reactivity
- Human
- Clone
- 3A5
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Chordoid Glioma and Papillary Glioneuronal Tumors.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA
- Gene ID
- 5578
- UniProt ID
- P17252
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2853 | Hi-Affi™ Recombinant Rabbit Anti-PRKCA Monoclonal Antibody (DS2853AB) | WB, IP, IHC-P, ICC/IF | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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