Rabbit Anti-PKN2 Recombinant Antibody (clone R02-5K8) (CAT#: VS3-XY3467)
The antibody is a recombinant rabbit antibody against human PKN2 (Protein kinase N2), which plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly and cell migration. The antibody also has cross-reactivity against mouse and rat PKN2. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/if_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/ihc_selected.jpg
Figure 3 IHC staining of human breast
(Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_2_4_selected.jpg
(Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_2_4_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_9_5.jpg
Figure 5 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_7_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_7_3.jpg
Figure 6 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_8_4.jpg
Figure 7 Testis
(Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_4_6.jpg
(Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_4_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065243-PKN2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065243-PKN2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PKN2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-5K8
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Renal Malformations; Bubonic Plague
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042
- Gene ID
- 5586
- UniProt ID
- Q16513
- Long Name
- Protein kinase N2
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- Cellular Localization
- Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus
- Post Translation Modifications
- Autophosphorylated. Phosphorylated during mitosis. Phosphorylated by CDK10 (PubMed:27104747).
Activated by limited proteolysis with trypsin (By similarity).
Proteolytically cleaved by caspase-3 during the induction of apoptotic cell death.
- Protein Refseq
- NP_001307636.1; NP_001307637.1; NP_001307638.1
- Function
- PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0782MZ | Recombinant Mouse Anti-Human PKN2 Antibody | WB | Mouse antibody |
| VS3-QX883 | Mouse Anti-PKN2 Recombinant Antibody (clone 4C2-B2-A0) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS4-CJ77 | Recombinant Rabbit Anti-PKN2 Antibody (clone 20A8) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| VS7-0425-WR622 | Mouse Anti-PKN2 Monoclonal Antibody (VS7-0425-WR622) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
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