Mouse Anti-NHP PKN2 Recombinant Antibody (clone 1D1)
CAT#: VS-1024-XY394
This expertly formulated mouse monoclonal antibody is specifically aimed at PKN2, exhibiting remarkable reactivity with human, mouse, non-human primate, and rat samples. Utilizing a purified recombinant fragment of human PKN2 as the immunogen, it is well-suited for a comprehensive range of applications, including Western blotting, immunohistochemistry, immunofluorescence, flow cytometry, and ELISA. Researchers can leverage this antibody to investigate the pivotal functions of PKN2 in cellular signaling and regulation, thereby enhancing our understanding of its contributions to various physiological processes and its potential implications in diverse pathological conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_2_4_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_8_4.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065243-PKN2
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Specifications
- Immunogen
- Purified recombinant fragment of human PKN2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PKN2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 1D1
- Applications
- WB, IHC, IF, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, IF: 1:100, FC: 1:200-1:400, ELISA: 1:8000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PKN2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0782MZ | Recombinant Mouse Anti-Human PKN2 Antibody | WB | Mouse antibody |
| VS3-QX883 | Mouse Anti-PKN2 Recombinant Antibody (clone 4C2-B2-A0) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS4-CJ77 | Recombinant Rabbit Anti-PKN2 Antibody (clone 20A8) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| VS3-XY3467 | Rabbit Anti-PKN2 Recombinant Antibody (clone R02-5K8) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS7-0425-WR622 | Mouse Anti-PKN2 Monoclonal Antibody (VS7-0425-WR622) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2727 | Hi-Affi™ Recombinant Rabbit Anti-PKN2 Monoclonal Antibody (DS2727AB) | WB, IHC-P, ICC, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1665 | Anti-PKN2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5449 | Anti-Human PKN2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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