Mouse Anti-NHP PKN2 Recombinant Antibody (clone 1D1) (CAT#: VS-1024-XY394)
This expertly formulated mouse monoclonal antibody is specifically aimed at PKN2, exhibiting remarkable reactivity with human, mouse, non-human primate, and rat samples. Utilizing a purified recombinant fragment of human PKN2 as the immunogen, it is well-suited for a comprehensive range of applications, including Western blotting, immunohistochemistry, immunofluorescence, flow cytometry, and ELISA. Researchers can leverage this antibody to investigate the pivotal functions of PKN2 in cellular signaling and regulation, thereby enhancing our understanding of its contributions to various physiological processes and its potential implications in diverse pathological conditions.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/if_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/ihc_selected.jpg
Figure 3 IHC staining of human breast
(Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_2_4_selected.jpg
(Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_2_4_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_9_5.jpg
Figure 5 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_7_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_7_3.jpg
Figure 6 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_8_4.jpg
Figure 7 Testis
(Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_4_6.jpg
(Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_4_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065243-PKN2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065243-PKN2
Specifications
- Immunogen
- Purified recombinant fragment of human PKN2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PKN2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 1D1
- Applications
- WB, IHC, IF, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, IF: 1:100, FC: 1:200-1:400, ELISA: 1:8000
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0782MZ | Recombinant Mouse Anti-Human PKN2 Antibody | WB | Mouse antibody |
| VS3-QX883 | Mouse Anti-PKN2 Recombinant Antibody (clone 4C2-B2-A0) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS4-CJ77 | Recombinant Rabbit Anti-PKN2 Antibody (clone 20A8) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| VS3-XY3467 | Rabbit Anti-PKN2 Recombinant Antibody (clone R02-5K8) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS7-0425-WR622 | Mouse Anti-PKN2 Monoclonal Antibody (VS7-0425-WR622) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
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