Rabbit Anti-POLR2A Recombinant Antibody (clone R06-8G7)
CAT#: VS3-FY1956
Clone R06-8G7 is a rabbit recombinant monoclonal antibody that has been developed to target human POLR2A. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. It is advised to dilute the sample for improved detectability by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The RNA polymerase II subunit B1 antibody is guaranteed for western blot analysis of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 lysates.
Tested Data
WB
Figure 1 Anti-POLR2A Antibody (VS3-FY1956) in WB.
The RNA polymerase II CTD repeat YSPTSPS expression in Hela, A549, MCF-7, U251, HL-60 lysates were detected using the anti-RNA polymerase II CTD repeat YSPTSPS by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human RNA polymerase II CTD repeat sequence YSPTSPS
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POLR2A
- Species Reactivity
- Human
- Clone
- R06-8G7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 217 kDa, while the observed molecular weight stands at 250 kDa.
- Related Disease
- Neurodevelopmental Disorder With Hypotonia And Variable Intellectual And Behavioral Abnormalities and Skull Base Meningioma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220
- Gene ID
- 5430
- UniProt ID
- P24928
- Sequence Similarities
- Belongs to the RNA polymerase beta' chain family.
- Cellular Localization
- Chromosome, Cytoplasm, DNA-directed RNA polymerase, Nucleus
- Post Translation Modifications
- The tandem heptapeptide repeats in the C-terminal domain (CTD) can be highly phosphorylated (PubMed:28076779).
The phosphorylation activates Pol II.
Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9.
CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA.
Phosphorylation also takes place at 'Ser-7' of the heptapeptide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts.
The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed.
Dephosphorylated by the protein phosphatase CTDSP1.
Dephosphorylated at 'Ser-2' following UV irradiation.
- Protein Refseq
- NP_000928.1
- Function
- Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "POLR2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2782 | Hi-Affi™ Recombinant Rabbit Anti-POLR2A Monoclonal Antibody (DS2782AB) | WB, IP, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2084 | PE-B*07:02/Human POLR2A (TPQSNRPVM) MHC Tetramer | FCM | |
| MHC-LC2085 | APC-B*07:02/Human POLR2A (TPQSNRPVM) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2362-CN | Recombinant Rabbit Anti-POLR2A (phosphorylated Ser2) Monoclonal Antibody (JM11-51) | WB, IP, IF, IHC, FC | Rabbit IgG |
| MRO-2363-CN | Recombinant Rabbit Anti-POLR2A (phosphorylated Ser5) Monoclonal Antibody (JM51-21) | WB, IP, IF, IHC, FC | Rabbit IgG |
| ZG-0514U | Rabbit Anti-Phospho-POLR2A (S2) Recombinant Antibody (clone 2G1) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS7-0425-WR646 | Mouse Anti-POLR2A Recombinant Antibody (clone 1A6B1) | WB, ELISA | Mouse IgG2b |
| VS7-0425-WR647 | Mouse Anti-POLR2A Recombinant Antibody (clone 1B1D8) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY213 | AbPlus™ Anti-POLR2A Magnetic Beads (JM11-51) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5551 | Anti-POLR2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY5552 | Anti-Human POLR2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY5553 | Anti-Yeast POLR2A Immunohistochemistry Kit | IHC |
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