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    Rabbit Anti-POU2AF1 Recombinant Antibody (clone R01-6M2) (CAT#: VS3-XY3473)

    This product is a recombinant rabbit antibody against POU2AF1 (POU class 2 homeobox associating factor 1). The antibody specifically reacts with human POU2AF1. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20, respectively.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IHC staining of human tonsil
    Figure 1 IHC staining of human tonsil
    (Immunohistochemical staining shows high expression in tonsil)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8_selected.jpg
    Normal Tissue
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27284_B_8_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_4_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_8.jpg
    Figure 8 Tonsil
    Figure 8 Tonsil
    (Germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Group enriched (Daudi, Karpas-707, U-266/70, U-266/84, U-698)
    Figure 9 RNA cell line category: Group enriched (Daudi, Karpas-707, U-266/70, U-266/84, U-698)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110777-POU2AF1/subcellular

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human POU2AF1
    • Species Reactivity
    • Human
    • Clone
    • R01-6M2
    • Applications
    • WB, IHC-P, IP
    • Related Disease
    • Primary Biliary Cholangitis; Mediastinal Gray Zone Lymphoma

    Product Property

    • Clonality
    • Monoclonal
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IHC: 1/50-1/100
      IP: 1/20

    Target

    • Alternative Names
    • BOB1; OBF1; OCAB; OBF-1
    • Long Name
    • POU class 2 homeobox associating factor 1
    • Sequence Similarities
    • Belongs to the POU2AF family.
    • Cellular Localization
    • Nucleus
    • Post Translation Modifications
    • Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • Function
    • Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290).
      It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176).
      It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176).
      It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290).
      Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    MOB-1507MZ Recombinant Mouse Anti-Human POU2AF1 Antibody (clone Xvf-BD6) IHC-P Mouse antibody
    ZG-0054J Mouse Anti-POU2AF1 Recombinant Antibody (ZG-0054J) IHC-P, IF Mouse IgG
    VS3-QX894 Mouse Anti-POU2AF1 Recombinant Antibody (clone 1170CT1.1.1) WB Mouse IgG1, κ
    VS3-WK1013 Mouse Anti-POU2AF1 Recombinant Antibody (VS3-WK1013) IHC Mouse IgG2a
    VS3-WK1014 Mouse Anti-POU2AF1 Recombinant Antibody (clone ABT-BOB1) IHC-P Mouse IgG2a, κ

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