Rabbit Anti-POU2AF1 Recombinant Antibody (clone R01-6M2)
CAT#: VS3-XY3473
This product is a recombinant rabbit antibody against POU2AF1 (POU class 2 homeobox associating factor 1). The antibody specifically reacts with human POU2AF1. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20, respectively.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human tonsil
Immunohistochemical staining shows high expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27284_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (Daudi, Karpas-707, U-266/70, U-266/84, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110777-POU2AF1/subcellular
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POU2AF1
- Species Reactivity
- Human
- Clone
- R01-6M2
- Applications
- WB, IHC-P, IP
- Related Disease
- Primary Biliary Cholangitis; Mediastinal Gray Zone Lymphoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- BOB1; OBF1; OCAB; OBF-1
- Gene ID
- 5450
- UniProt ID
- Q16633
- Long Name
- POU class 2 homeobox associating factor 1
- Sequence Similarities
- Belongs to the POU2AF family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
- Protein Refseq
- NP_006226.2
- Function
- Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290).
It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176).
It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176).
It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290).
Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3473. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "POU2AF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2794 | Hi-Affi™ Recombinant Rabbit Anti-POU2AF1 Monoclonal Antibody (DS2794AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1934 | PE-B*51:01/Human POU2AF1 (DAYALNHTL) MHC Tetramer | FCM | |
| MHC-LC4449 | PE-B*15:01/Human POU2AF1 (LPHQPLATY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0054J | Mouse Anti-POU2AF1 Recombinant Antibody (ZG-0054J) | IHC-P, IF | Mouse IgG |
| ZG-0516U | Rabbit Anti-POU2AF1 Recombinant Antibody (clone 4A5) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX894 | Mouse Anti-POU2AF1 Recombinant Antibody (clone 1170CT1.1.1) | WB | Mouse IgG1, κ |
| VS3-WK1013 | Mouse Anti-POU2AF1 Recombinant Antibody (VS3-WK1013) | IHC | Mouse IgG2a |
| VS3-FY1168 | Recombinant Mouse Anti-POU2AF1 Antibody (clone 4G9-4A8-3E8) | IHC-P | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC919 | CytoStream™ Rabbit Anti-POU2AF1 Recombinant Antibody (VS13-YC919) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5579 | Anti-POU2AF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5580 | Anti-Mouse POU2AF1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Block, Cyt, FuncS, Inhib
Application: WB, ELISA, FuncS
Application: WB, ELISA, IF
Application: ELISA, IHC, FC, IP, IF, BL
Application: ELISA, IHC, FC, IP, IF, Inhib
-3.jpg)
Application: ELISA, WB
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
