Rabbit Anti-POU2F2 Recombinant Antibody (clone R07-8H6)
CAT#: VS3-FY1629
This anti-Oct-2 antibody detected positive in western blot analysis of 44106 in rat brain lysates. The recombinant monoclonal antibody has been developed to target human and rat POU2F2. The R07-8H6 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49056/1533_E11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64404/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64404/147786_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_7_8.jpg
Normal Tissue
Figure 7 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2513/6476_A_6_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000028277-POU2F2/subcellular
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WB
Figure 1 Anti-POU2F2 Antibody (VS3-FY1629) in WB.
The Oct-2 expression in rat Brain lysates were detected using the anti-Oct-2/POU2F2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Oct-2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat POU2F2
- Species Reactivity
- Human, Rat
- Clone
- R07-8H6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 51 kDa, while the observed molecular weight stands at 55 kDa.
- Related Disease
- B-Cell Lymphoma and Papilloma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- OCT2; OTF2; Oct-2
- Gene ID
- 5452
- UniProt ID
- P09086
- Sequence Similarities
- Belongs to the POU transcription factor family. Class-2 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_001193954.1; NP_001193955.1; NP_001234923.1N
- Function
- Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3').
Regulates IL6 expression in B cells with POU2AF1 (By similarity).
Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.
Modulates transcription transactivation by NR3C1, AR and PGR.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "POU2F2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2795 | Hi-Affi™ Recombinant Rabbit Anti-POU2F2 Monoclonal Antibody (DS2795AB) | FC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ532 | Mouse Anti-POU2F2 Recombinant Antibody (clone PT0202) | IHC-P | Mouse IgG1, κ |
| VS3-CJ533 | Mouse Anti-POU2F2 Recombinant Antibody (clone ABT-OCT2) | IHC-P | Mouse IgG |
| VS3-FY1170 | Recombinant Mouse Anti-POU2F2 Antibody (clone 2B8-2E6-3A8) | IHC-P | Mouse IgG1 |
| VS3-FY1171 | Recombinant Rabbit Anti-POU2F2 Antibody (clone R02-5E7) | WB | Rabbit IgG |
| VS3-XY3474 | Rabbit Anti-POU2F2 Recombinant Antibody (clone R09-8M8) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5582 | Anti-POU2F2 Immunohistochemistry Kit | IHC |
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