Recombinant Mouse Anti-POU2F2 Antibody (clone 2B8-2E6-3A8) (CAT#: VS3-FY1170)

• Immunogen
• Synthetic peptide derived from human Oct-2.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human POU2F2

• Species Reactivity
• Human

• Clone
• 2B8-2E6-3A8

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS, pH 7.3, 50% glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry-Paraffin: 1/100-1/500

• Alternative Names
• POU2F2; OCT2; OTF2; POU domain; class 2; transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2

• Gene ID
• 5452

• UniProt ID
• P09086

• Sequence Similarities
• Belongs to the POU transcription factor family. Class-2 subfamily.

• Cellular Localization
• Cytoplasm, Nucleus

• Protein Refseq
• NP_001193954.1; NP_001193955.1; NP_001234923.1N

• Function
• Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290).
  Regulates IL6 expression in B cells with POU2AF1 (By similarity).
  Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654).
  Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874).

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS