Recombinant Mouse Anti-POU2F2 Antibody (clone 2B8-2E6-3A8)

CAT#: VS3-FY1170

This product is a recombinant mouse antibody that recognizes POU2F2. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 2B8-2E6-3A8 is specific for human POU2F2. The antigen is synthetic peptide derived from human oct-2.

Gene Expression Tested Data
Figure 1 IF staining of human cell line BJ Figure 2 IHC staining of human tonsil Figure 3 Cerebral cortex Figure 4 Colon Figure 5 Testis Figure 6 Lymph node Figure 7 Tonsil Figure 8 RNA cell line category: Cell line enhanced.
Figure 1. Recombinant Mouse Anti-POU2F2 Antibody (clone 2B8-2E6-3A8) in IHC-P.

Specifications

  • Immunogen
  • Synthetic peptide derived from human Oct-2.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human POU2F2
  • Species Reactivity
  • Human
  • Clone
  • 2B8-2E6-3A8
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity Chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS, pH 7.3, 50% glycerol, 0.5% BSA
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Immunohistochemistry-Paraffin: 1/100-1/500

Target

  • Alternative Names
  • POU2F2; OCT2; OTF2; POU domain; class 2; transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2
  • Sequence Similarities
  • Belongs to the POU transcription factor family. Class-2 subfamily.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Function
  • Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290).
    Regulates IL6 expression in B cells with POU2AF1 (By similarity).
    Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654).
    Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874).
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Secondary Antibody

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Reconstitution Buffer

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VS-0525-XY5582 Anti-POU2F2 Immunohistochemistry Kit IHC
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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