Mouse Anti-POU5F1 Recombinant Antibody (clone 3F5-F9-E5)
CAT#: VS3-QX895
This product is a mouse antibody that recognizes POU5F1. The antibody can be used in applications: WB.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular
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Specifications
- Immunogen
- Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- POU5F1/OCT4
- Species Reactivity
- Mouse
- Clone
- 3F5-F9-E5
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl), 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "POU5F1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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