Mouse Anti-POU5F1 Recombinant Antibody (ZG-0325C) (CAT#: ZG-0325C)

Name: Mouse Anti-POU5F1 Recombinant Antibody (ZG-0325C)
SKU: ZG-0325C
Rating: 5 (1 reviews)

This product is a mouse antibody that recognizes POU5F1. It can be used in a wide variety of applications, such as: WB, ELISA.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Mouse Anti-POU5F1 Antibody (ZG-0325C) in WB

Figure 1 Mouse Anti-POU5F1 Antibody (ZG-0325C) in WB

Western Blot analysis using Oct-3/4 monoclonal antibody against NTERA-2 cell lysate (1).

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg

Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg

Normal Tissue

Figure 3 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg

Figure 4 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg

Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg

Figure 6 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg

Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg

Figure 8 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg

Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg

RNA Expression

Figure 10 RNA cell line category: Cell line enriched (SuSa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthesized peptide of human Oct-3/4.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human POU5F1

Species Reactivity
Human

Applications
WB, ELISA

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
1 mg/mL

Storage
Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
WB 1:500-1:2000. ELISA 1:10000.

Target

Alternative Names
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4

Gene ID
5460

UniProt ID
Q01860

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application	Type
MOB-3985z	Mouse Anti-POU5F1 Recombinant Antibody (clone 43H9)	WB, IP, IHC, ELISA	Mouse IgG
VS3-QX895	Mouse Anti-POU5F1 Recombinant Antibody (clone 3F5-F9-E5)	WB	Mouse IgG2b
VS3-XY1279	Mouse Anti-POU5F1 Recombinant Antibody (clone 1D10H6)	ELISA, WB	Mouse IgG1
VS3-XY1280	Mouse Anti-POU5F1 Recombinant Antibody (clone 3A5)	ELISA, WB	Mouse IgG1
VS11-QX120	Mouse Anti-POU5F1 Recombinant Antibody (clone 41B1)	IHC, WB	Mouse IgG2b, κ

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