Mouse Anti-PPARG Monoclonal Antibody (clone 3A4A9)
CAT#: VS7-0425-WR656
This product is a mouse IgG1 antibody that binds to human PPARG. The antibody 3A4A9 recognizes specific amino acid domain of PPARG (aa170-270). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
Normal Tissue
Figure 6 Placenta
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BEWO, CAPAN-2, HSkMC, PC-3, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
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Specifications
- Immunogen
- Recombinant PPARG (aa170-270) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PPARG
- Species Reactivity
- Human
- Clone
- 3A4A9
- Molecule Class
- Peroxisome Proliferator-Activated Receptor (Ppar) Nuclear Receptor
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Obesity (OBESITY); Lipodystrophy, familial partial, 3 (FPLD3); Glioma 1 (GLM1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PPARG; Peroxisome Proliferator-Activated Nuclear Receptor Gamma Variant 1; GLM1; PPAR-Gamma; PPARG2; CIMT1; Peroxisome Proliferative Activated Receptor, Gamma; Peroxisome Proliferator-Activated Receptor Gamma; PPARG1; NR1C3
- Gene ID
- 5468
- UniProt ID
- P37231
- Long Name
- Peroxisome proliferator activated receptor gamma
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Glycoprotein; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001317544.2
- Function
- Activator; DNA-binding; Receptor
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Related Signaling Pathways
mTOR Signaling Pathway
Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPARG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1668z | Mouse Anti-PPARG Recombinant Antibody (clone 28C5) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-2265CT | Recombinant Mouse anti-Human PPARG Monoclonal antibody (TQN302) | IHC-P, WB, ELISA, IP, ChIP, GSA | |
| MRO-1258-CN | Recombinant Rabbit Anti-PPARG Monoclonal Antibody (JF101-4) | WB | Rabbit IgG |
| ZG-0388C | Mouse Anti-PPARG Recombinant Antibody (ZG-0388C) | WB, IF | Mouse IgG |
| ZG-0389C | Mouse Anti-PPARG Recombinant Antibody (ZG-0389C) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0429MZ | Chicken Anti-PPARγ Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1705 | Anti-PPARG Immunohistochemistry Kit | IHC | |
| VS-0525-XY5591 | Anti-Human PPARG Immunohistochemistry Kit | IHC |
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