Mouse Anti-PPARG Monoclonal Antibody (clone 3A4A9) (CAT#: VS7-0425-WR656)
This product is a mouse IgG1 antibody that binds to human PPARG. The antibody 3A4A9 recognizes specific amino acid domain of PPARG (aa170-270). It can be used for WB and ELISA assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
Figure 2 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
(Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
Figure 6 Placenta
(Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
(Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BEWO, CAPAN-2, HSkMC, PC-3, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
Specifications
- Immunogen
- Recombinant PPARG (aa170-270) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PPARG
- Species Reactivity
- Human
- Clone
- 3A4A9
- Molecule Class
- Peroxisome Proliferator-Activated Receptor (Ppar) Nuclear Receptor
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Obesity (OBESITY); Lipodystrophy, familial partial, 3 (FPLD3); Glioma 1 (GLM1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PPARG; Peroxisome Proliferator-Activated Nuclear Receptor Gamma Variant 1; GLM1; PPAR-Gamma; PPARG2; CIMT1; Peroxisome Proliferative Activated Receptor, Gamma; Peroxisome Proliferator-Activated Receptor Gamma; PPARG1; NR1C3
- Gene ID
- 5468
- UniProt ID
- P37231
- Long Name
- Peroxisome proliferator activated receptor gamma
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Glycoprotein; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001317544.2
- Function
- Activator; DNA-binding; Receptor
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
mTOR Signaling Pathway
Throid Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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