Mouse Anti-PPP2CA Recombinant Antibody (VS7-0425-WR666) (CAT#: VS7-0425-WR666)
This product is a mouse IgG antibody that binds to human PPP2CA. The antibody can be used for WB, IF, and ICC assays. It cross reacts with mouse, bovine, chicken, dog, pig, rabbit, and Zebrafish.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_B_9_5.jpg
Figure 3 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_3.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_3.jpg
Figure 4 Liver
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_4.jpg
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_5.jpg
(Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_5_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_5_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113575-PPP2CA/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113575-PPP2CA/subcellular
Specifications
- Immunogen
- Recombinant human PPP2CA (N-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPP2CA
- Species Reactivity
- Human, Mouse, Bovine, Chicken, Dog, Pig, Rabbit, Zebrafish
- Molecule Class
- Serine/Threonine Protein Phosphatase
- Applications
- WB, IF, ICC
- Conjugate
- Unconjugated
- Related Disease
- Houge-Janssens syndrome 3 (HJS3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Immunofluorescence: 1:100 - 1:500
Target
- Alternative Names
- HJS3; RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha
- Gene ID
- 5515
- UniProt ID
- P67775
- Long Name
- Protein Phosphatase 2 Catalytic Subunit Alpha
- Cellular Localization
- Centromere; Chromosome; Cytoplasm; Cytoskeleton; Nucleus
- Post Translation Modifications
- Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001341948.1
- Function
- Hydrolase; Protein phosphatase
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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