Mouse Anti-PPP2CA Recombinant Antibody (VS7-0425-WR666)
CAT#: VS7-0425-WR666
This product is a mouse IgG antibody that binds to human PPP2CA. The antibody can be used for WB, IF, and ICC assays. It cross reacts with mouse, bovine, chicken, dog, pig, rabbit, and Zebrafish.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113575-PPP2CA/subcellular
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Specifications
- Immunogen
- Recombinant human PPP2CA (N-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPP2CA
- Species Reactivity
- Human, Mouse, Bovine, Chicken, Dog, Pig, Rabbit, Zebrafish
- Molecule Class
- Serine/Threonine Protein Phosphatase
- Applications
- WB, IF, ICC
- Conjugate
- Unconjugated
- Related Disease
- Houge-Janssens syndrome 3 (HJS3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Immunofluorescence: 1:100 - 1:500
Target
- Alternative Names
- HJS3; RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha
- Gene ID
- 5515
- UniProt ID
- P67775
- Long Name
- Protein Phosphatase 2 Catalytic Subunit Alpha
- Cellular Localization
- Centromere; Chromosome; Cytoplasm; Cytoskeleton; Nucleus
- Post Translation Modifications
- Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001341948.1
- Function
- Hydrolase; Protein phosphatase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPP2CA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC159 | PE-A*02:01/Human Ser/Thr protein phosphatase 2A (SLLPAIVEL) MHC Tetramer | FCM | |
| MHC-LC160 | APC-A*02:01/Human Ser/Thr Protein phosphatase 2A (SLLPAIVEL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2825 | Hi-Affi™ Recombinant Rabbit Anti-PPP2CA Monoclonal Antibody (DS2825AB) | WB, FC, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0518U | Rabbit Anti-Phospho-PPP2CA (Y307) Recombinant Antibody (clone 3F11) | ELISA, WB | Rabbit IgG |
| VS1-YJ181 | Mouse Anti-PPP2CA Recombinant Antibody (clone 2G9) | WB, IP | Mouse IgG1 |
| VS1-YJ182 | Mouse Anti-PPP2CA Recombinant Antibody (clone 2G9), HRP | WB, IP | Mouse IgG1 |
| VS1-YJ187 | Mouse Anti-PPP2CA Recombinant Antibody (clone 7A6) | IP, WB | Mouse IgG1 |
| VS1-YJ188 | Mouse Anti-PPP2CA Recombinant Antibody (clone 7A6), HRP | IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY214 | AbPlus™ Anti-PPP2CA Magnetic Beads (7A6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1723 | Anti-PPP2CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5629 | Anti-Mouse PPP2CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5628 | Anti-Human PPP2CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5630 | Anti-Bovine PPP2CA Immunohistochemistry Kit | IHC |
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