Rabbit Anti-PPP4C Recombinant Antibody (clone R05-4F9) (CAT#: VS3-FY1608)

• Immunogen
• Recombinant human PPP4C protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat PPP4C

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R05-4F9

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 35 kDa, while the observed molecular weight stands at 35 kDa.

• Related Disease
• Contagious Pustular Dermatitis and Severe Combined Immunodeficiency

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• PP-X, PP4, PP4C, PPH3, PPP4, PPX

• Gene ID
• 5531

• UniProt ID
• P60510

• Sequence Similarities
• Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

• Cellular Localization
• Nucleus, Cytoplasm, Cytoskeleton

• Post Translation Modifications
• Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.

• Protein Refseq
• NP_001290432.1; NP_001290433.1; NP_001290435.1

• Function
• Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).
  In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody