Mouse Anti-PRDM1 Recombinant Antibody (clone 2F1B6) (CAT#: VS7-0425-WR673)

• Immunogen
• Recombinant human PRDM1 (AA: 690-825) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human PRDM1

• Species Reactivity
• Human

• Clone
• 2F1B6

• Molecule Class
• Zinc-Finger Protein

• Applications
• ICC, FC

• Conjugate
• Unconjugated

• Related Disease
• In certain aggressive cases of activated B cell-like diffuse large B-cell lymphoma (ABC-DLBCL), PRDM1 protein instability has been observed. This instability, which impairs B-cell differentiation, is caused by N-terminal misfolding mutations, including those occurring at positions Pro-84 and Ile-107, and results in PRDM1 protein sequestration in the cytoplasm, followed by proteasomal degradation via a heat shock protein 70 HSPA1A-SYNV1/HRD1 pathway. These N-terminal mutations do not affect PRDM1 transcription regulation activity. HSPA1A inhibition restores PRDM1 nuclear localization and transcriptional activity in lymphoma cell lines and suppresses tumor growth in xenografts, more efficiently than proteasome inhibition

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunocytochemistry: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• BLIMP1; BLIMP-1; PRDI-BF1

• Gene ID
• 639

• UniProt ID
• O75626

• Long Name
• PR/SET domain 1

• Cellular Localization
• Cytoplasm; Nucleus

• Post Translation Modifications
• Isopeptide bond; Ubl conjugation

• Protein Refseq
• NP_001189.2

• Function
• DNA-binding; Methyltransferase; Repressor; Transferase

Isotype Control

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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