Rabbit Anti-PRDX3 Recombinant Antibody (clone R07-3I3) (CAT#: VS3-FY2061)
The clone R07-3I3 is a rabbit recombinant monoclonal antibody that binds specifically to human PRDX3. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. The dilution ratio that is advised for optimal results is as follows: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
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ICC
Figure 1 Anti-PRDX3 Antibody (VS3-FY2061) in ICC.
Immunocytochemistry staining of Peroxiredoxin 3 (green) in Hela using the anti-Peroxiredoxin 3 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-PRDX3 Antibody (VS3-FY2061) in WB.
The Peroxiredoxin 3/PRDX3 expression in Hela, HL-60, lysates were detected using the anti-Peroxiredoxin 3/PRDX3 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human peroxiredoxin 3/PRDX3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRDX3
- Species Reactivity
- Human
- Clone
- R07-3I3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 28 kDa, while the observed molecular weight stands at 28 kDa.
- Related Disease
- Spinocerebellar Ataxia, Autosomal Recessive 32 and Corneal Dystrophy, Punctiform And Polychromatic Pre-Descemet
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PRDX3; AOP-1; AOP1; HBC189; MER5; PRO1748; SP-22; prx-III; peroxiredoxin 3
- Gene ID
- 10935
- UniProt ID
- P30048
- Sequence Similarities
- Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
- Cellular Localization
- Cytoplasm, Endosome, Mitochondrion
- Post Translation Modifications
- Phosphorylated by LRRK2; phosphorylation reduces perodixase activity., The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond., S-palmitoylated.
- Protein Refseq
- NP_001289201.1; NP_006784.1;
- Function
- Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.
Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Required for the maintenance of physical strength (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0437MZ | Chicken Anti-PRDX3 Polyclonal IgY | WB | Chicken antibody |
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