Rabbit Anti-PRDX3 Recombinant Antibody (clone R07-3I3)
CAT#: VS3-FY2061
The clone R07-3I3 is a rabbit recombinant monoclonal antibody that binds specifically to human PRDX3. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. The dilution ratio that is advised for optimal results is as follows: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79909/1987_C10_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8656/23380_B_8_5.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8656/23025_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165672-PRDX3/subcellular
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ICC
Figure 1 Anti-PRDX3 Antibody (VS3-FY2061) in ICC.
Immunocytochemistry staining of Peroxiredoxin 3 (green) in Hela using the anti-Peroxiredoxin 3 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-PRDX3 Antibody (VS3-FY2061) in WB.
The Peroxiredoxin 3/PRDX3 expression in Hela, HL-60, lysates were detected using the anti-Peroxiredoxin 3/PRDX3 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human peroxiredoxin 3/PRDX3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRDX3
- Species Reactivity
- Human
- Clone
- R07-3I3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 28 kDa, while the observed molecular weight stands at 28 kDa.
- Related Disease
- Spinocerebellar Ataxia, Autosomal Recessive 32 and Corneal Dystrophy, Punctiform And Polychromatic Pre-Descemet
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PRDX3; AOP-1; AOP1; HBC189; MER5; PRO1748; SP-22; prx-III; peroxiredoxin 3
- Gene ID
- 10935
- UniProt ID
- P30048
- Sequence Similarities
- Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
- Cellular Localization
- Cytoplasm, Endosome, Mitochondrion
- Post Translation Modifications
- Phosphorylated by LRRK2; phosphorylation reduces perodixase activity., The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond., S-palmitoylated.
- Protein Refseq
- NP_001289201.1; NP_006784.1;
- Function
- Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.
Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Required for the maintenance of physical strength (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRDX3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3766z | Mouse Anti-PRDX3 Recombinant Antibody (clone 3F3) | WB, ELISA, ICC, IF, IP | Mouse IgG1, κ |
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| VS4-CJ87 | Recombinant Rabbit Anti-PRDX3 Antibody (clone 14E2) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| VS4-CJ88 | Recombinant Mouse Anti-PRDX3 Antibody (clone 12A10C12) | ELISA, WB, IHC, IF, FC | Mouse IgG2b |
| VS4-CJ89 | Recombinant Mouse Anti-PRDX3 Antibody (clone 6E6E12) | ELISA, WB, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0437MZ | Chicken Anti-PRDX3 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2838 | Hi-Affi™ Recombinant Rabbit Anti-PRDX3 Monoclonal Antibody (DS2838AB) | WB, IHC, ICC, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC927 | CytoStream™ Rabbit Anti-PRDX3 Recombinant Antibody (VS13-YC927) | WB, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1734 | Anti-PRDX3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5657 | Anti-Mouse PRDX3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5656 | Anti-Human PRDX3 Immunohistochemistry Kit | IHC |
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