Mouse Anti-PRKAB1 Recombinant Antibody (clone 5D8)
CAT#: VS3-WK1595
This antibody is a mouse recombinant antibody against human PRKAB1, which may serve as an adaptor molecule mediating the association of the AMPK complex. The clone 5D8 can be used in IHC analysis at a dilution of 1/50-1/200.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4247/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111725-PRKAB1/subcellular
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Specifications
- Immunogen
- Synthesized peptide derived from human AMPKβ1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PRKAB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5D8
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC: 1/50-1/200
Target
- Alternative Names
- AMPK; HAMPKb
- Gene ID
- 5564
- UniProt ID
- Q9Y478
- Long Name
- Protein kinase AMP-activated non-catalytic subunit beta 1
- Sequence Similarities
- Belongs to the 5'-AMP-activated protein kinase beta subunit family.
- Cellular Localization
- Cytoplasm, Cytosol, Nucleoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
- Protein Refseq
- NP_006244.2
- Function
- Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "PRKAB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2846 | Hi-Affi™ Recombinant Rabbit Anti-PRKAB1 Monoclonal Antibody (DS2846AB) | WB, FC | IgG |
| MOR-4673 | Hi-Affi™ Recombinant Rabbit Anti-PRKAB1 Monoclonal Antibody (TH187DS) | WB, IF, ICC, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0091-CN | Recombinant Rabbit Anti-PRKAB1 Monoclonal Antibody (CBACN-026) | WB, FC | Rabbit IgG |
| ZG-0286F | Mouse Anti-PRKAB1 Recombinant Antibody (ZG-0286F) | WB, ICC | Mouse IgG |
| VS3-QX916 | Mouse Anti-PRKAB1 Recombinant Antibody (clone 1A7-E11-E9) | WB, ICC, IP, IHC | Mouse IgG2a |
| VS3-WK1225 | Mouse Anti-PRKAB1 Recombinant Antibody (VS3-WK1225) | WB | Mouse IgG |
| VS3-WK1226 | Rabbit Anti-PRKAB1 Recombinant Antibody (VS3-WK1226) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC929 | CytoStream™ Rabbit Anti-PRKAB1 Polyclonal Antibody (VS13-YC929) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5674 | Anti-PRKAB1 Immunohistochemistry Kit | IHC |
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