CytoStream™ Rabbit Anti-PTK2 Recombinant Antibody (VS13-YC956)
CAT#: VS13-YC956
The CytoStream™ Rabbit Anti-PTK2 Recombinant Antibody is specific for human and mouse PTK2. PTK2 encodes a tyrosine kinase found at cell adhesion sites, involved in growth and signal transduction triggered by extracellular interactions. The anti-PTK2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_6.jpg
Normal Tissue
Figure 8 Breast
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Myoepithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_1_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169398-PTK2/subcellular
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Specifications
- Immunogen
- Recombinant human FAK, aa 700-740.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PTK2
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC, IP
- Conjugate
- NONE
- Related Disease
- Cardiovascular Disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PTK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2031z | Mouse Anti-PTK2 Recombinant Antibody (clone 11B8) | WB, ELISA, ICC, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0669CT | Recombinant Mouse anti-Human PTK2 Monoclonal antibody (N232) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0748MZ | Chicken Anti-FAK1 Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2924 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (DS2924AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4573 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH85DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4678 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH192DS) | WB, IF, ICC, IHC-P, FC, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4679 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH193DS) | IF, ICC, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4680 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH194DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2719 | PE-A*02:01/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2720 | PE-A*02:07/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4514 | PE-Mamu-A1*001:01/Human PTK2 (LTAAHALAV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0564-CN | Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (CBACN-222) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2325-CN | Recombinant Rabbit Anti-PTK2 (phosphorylated Tyr397) Monoclonal Antibody (CBACN-616) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0329J | Mouse Anti-PTK2 Recombinant Antibody (ZG-0329J) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY219 | AbPlus™ Anti-PTK2 Magnetic Beads (CBACN-222) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5845 | Anti-PTK2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5846 | Anti-Mouse PTK2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5847 | Anti-Rat PTK2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL72 | Rabbit Anti-FAK (phospho Y397) Monoclonal Antibody (RCB-P0983) | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL164 | Rabbit Anti-FAK (phospho Y397) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
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