Mouse Anti-PTK2B Recombinant Antibody (clone 4B4-D0-F3)

Name: Mouse Anti-PTK2B Recombinant Antibody (clone 4B4-D0-F3)
SKU: VS3-QX937
Rating: 5 (1 reviews)

CAT#: VS3-QX937

This product is a mouse antibody that recognizes PTK2B. The antibody can be used in applications: WB, IHC, ICC, FC, ELISA.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3850/624_D4_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/ihc_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_B_7_5.jpg

Normal Tissue Figure 4 Colon

Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_3.jpg

Normal Tissue Figure 5 Liver

Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Hepatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_4.jpg

Normal Tissue Figure 6 Kidney

Cells in glomeruli
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_5.jpg

Normal Tissue Figure 7 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_4_6.jpg

Normal Tissue Figure 8 Tonsil

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Squamous epithelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_6_8.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_9_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (Daudi, REH)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000120899-PTK2B/subcellular

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Specifications

Immunogen
Purified recombinant fragment of PYK2 (aa815-997) expressed in E. coli.

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG2a

Specificity
Pyk2

Species Reactivity
Human

Clone
4B4-D0-F3

Applications
WB, IHC, ICC, FC, ELISA

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000

Target

Alternative Names
CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK

Gene ID
2185

UniProt ID
Q14289

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-QX937.pdf

MSDS

MSDS - VS3-QX937.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

See other products for "PTK2B"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1733CT	Recombinant Mouse anti-Human PTK2B Monoclonal antibody (EML069)	WB
MRO-1301-CN	Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (CBACN-469)	WB, IF, IHC	Rabbit IgG
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VS7-0425-WR714	Mouse Anti-PTK2B Recombinant Antibody (VS7-0425-WR714)	WB	Mouse IgG
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CAT	Product Name	Application	Type
BRD-0754MZ	Chicken Anti-FAK2 Polyclonal IgY	ELISA, IHC, IP, WB	Chicken antibody

CAT	Product Name	Application	Type
MOR-2925	Hi-Affi™ Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (DS2925AB)	WB, IP	IgG
MOR-4574	Hi-Affi™ Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (TH86DS)	WB, IF, ICC, IHC-P, FC, ChIP	IgG
MOR-4681	Hi-Affi™ Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (TH195DS)	WB, ChIP	IgG

CAT	Product Name	Application	Type
HPAB-1397-FY-F(E)	Mouse Anti-PTK2B Recombinant Antibody; Fab Fragment (HPAB-1397-FY-F(E))	WB, FC	Mouse Fab

CAT	Product Name	Application	Type
HPAB-1397-FY-S(P)	Mouse Anti-PTK2B Recombinant Antibody; scFv Fragment (HPAB-1397-FY-S(P))	WB, FC	Mouse scFv

CAT	Product Name	Application
VS-0325-XY1818	Anti-PTK2B Immunohistochemistry Kit	IHC
VS-0525-XY5849	Anti-Mouse PTK2B Immunohistochemistry Kit	IHC
VS-0525-XY5848	Anti-Human PTK2B Immunohistochemistry Kit	IHC

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