Rabbit Anti-PTPN1 Recombinant Antibody (clone 1G3) (CAT#: ZG-0532U)
Rabbit Anti-PTPN1 Antibody (clone 1G3) displayed high specificity against the PTPN1 protein of Human.
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Custom Production
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WB
Figure 1 Rabbit Anti-PTPN1 Antibody (ZG-0532U) in WB
Western Blot
Positive WB detected in: PC-3 whole cell lysate, THP-1 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, U87 whole cell lysate
All lanes: PTPN1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
IHC
Figure 2 Rabbit Anti-PTPN1 Antibody (ZG-0532U) in IHC
IHC image of ZG-0532U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-PTPN1 Antibody (ZG-0532U) in IHC
IHC image of ZG-0532U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PTP1B
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PTPN1
- Species Reactivity
- Human
- Clone
- 1G3
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Type 2 Diabetes Mellitus and Bubonic Plague.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Tyrosine-protein phosphatase non-receptor type 1; EC 3.1.3.48; Protein-tyrosine phosphatase 1B; PTP-1B; PTPN1; PTP1B
- Gene ID
- 5770
- UniProt ID
- P18031
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0801CT | Recombinant Mouse anti-Human PTPN1 Monoclonal antibody (208BU700) | ELISA, WB | |
| HPAB-0374-CN | Chicken Anti-PTPN1 Recombinant Antibody (HPAB-0374-CN) | WB, ELISA, IP | Chicken IgY |
| MRO-2145-CN | Rabbit Anti-PTPN1 Polyclonal Antibody (MRO-2145-CN) | WB, IF, IHC | Rabbit IgG |
| MRO-2146-CN | Rabbit Anti-PTPN1 Polyclonal Antibody (MRO-2146-CN) | WB, IF, IHC | Rabbit IgG |
| VS7-0425-WR717 | Mouse Anti-PTPN1 Recombinant Antibody (clone 4F11B9) | WB, FC | Mouse IgG1 |
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