Camelid Anti-PYCARD Recombinant Single Domain Antibody (Clone VHHASC)
CAT#: PABS-0127
This recombinant monoclonal antibody to human PYCARD is a Camelid monoclonal antibody that can be used for applications: ELISA, Block.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15948/36925_B_7_4_selected.jpg
Normal Tissue
Figure 3 Colon
Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_3.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (MCF7, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103490-PYCARD/subcellular
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Specifications
- Immunogen
- MBP-ASC
- Host Species
- Camelid
- Derivation
- Phage display library
- Type
- Camelid VHH
- Specificity
- Human PYCARD
- Species Reactivity
- Human
- Clone
- VHHASC
- Applications
- ELISA, Block, FuncS
- Related Disease
- Chronic recurrent multifocal osteomyelitis, Muckle-Wells syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Ideal for Apoptosis Research
Using the Camelid Anti-PYCARD Antibody has significantly enhanced our apoptosis research. Its specificity to PYCARD allows us to track and analyze apoptotic bodies with increased precision, making it a valuable instrument in understanding cell death pathways.
Enhances Understanding of Immune Mechanisms
Utilizing this antibody has enriched our understanding of immune mechanisms via PYCARD signaling. It offers a clear window into how cells communicate signals of death and survival, further elucidating core immune functions.
Effective Research Tool
I recently used the Camelid Anti-PYCARD Recombinant Single Domain Antibody for my research on inflammation pathways, and found it to be a robust and reliable tool. The high sensitivity and specificity were especially beneficial for my experiments, enabling clear and reproducible results. It's a significant addition to my lab resources.
Q&As
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Is this antibody functional in immunoprecipitation protocols?
A: While primarily used for ELISA and blocking, it may perform in immunoprecipitation; however, specific protocol optimization might be required to ensure effectiveness.
-
Why is this antibody a good choice for studying apoptosis?
A: PYCARD is a key adaptor protein in apoptosis signaling, and this antibody helps elucidate the role of PYCARD in cell death pathways due to its specificity and binding efficiency.
-
Is PYCARD expression detectable in non-cancerous tissues with this antibody?
A: Yes, this antibody can be utilized to detect PYCARD in various tissue samples, including immune-related structures, supporting studies on basal expression and pathogenesis.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PYCARD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2948 | Hi-Affi™ Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (DS2948AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1506-CN | Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (CBACN-553) | WB, IF, FC | Rabbit IgG |
| VS3-WK1213 | Rabbit Anti-PYCARD Recombinant Antibody (VS3-WK1213) | WB, ICC, IF, FC | Rabbit IgG |
| VS7-0425-WR728 | Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D3) | IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR729 | Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D7) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC807 | AbPlus™ Anti-PYCARD Magnetic Beads (VS-0724-YC807) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1834 | Anti-PYCARD Immunohistochemistry Kit | IHC | |
| VS-0525-XY5896 | Anti-Human PYCARD Immunohistochemistry Kit | IHC |
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