Camelid Anti-PYCARD Recombinant Single Domain Antibody (Clone VHHASC) (CAT#: PABS-0127)
This recombinant monoclonal antibody to human PYCARD is a Camelid monoclonal antibody that can be used for applications: ELISA, Block.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/if_selected.jpg
Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15948/36925_B_7_4_selected.jpg
(Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15948/36925_B_7_4_selected.jpg
Figure 3 Colon
(Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_3.jpg
(Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_3.jpg
Figure 4 Lymph node
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_8.jpg
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_8.jpg
Figure 5 Spleen
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_B_7_4.jpg
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_B_7_4.jpg
Figure 6 RNA cell line category: Cell line enhanced (MCF7, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103490-PYCARD/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103490-PYCARD/subcellular
Specifications
- Immunogen
- MBP-ASC
- Host Species
- Camelid
- Derivation
- Phage display library
- Type
- Camelid VHH
- Specificity
- Human PYCARD
- Species Reactivity
- Human
- Clone
- VHHASC
- Applications
- ELISA, Block, FuncS
- Related Disease
- Chronic recurrent multifocal osteomyelitis, Muckle-Wells syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2948 | Hi-Affi™ Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (DS2948AB) | WB, IP, FC | IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Ideal for Apoptosis Research
Using the Camelid Anti-PYCARD Antibody has significantly enhanced our apoptosis research. Its specificity to PYCARD allows us to track and analyze apoptotic bodies with increased precision, making it a valuable instrument in understanding cell death pathways.
Enhances Understanding of Immune Mechanisms
Utilizing this antibody has enriched our understanding of immune mechanisms via PYCARD signaling. It offers a clear window into how cells communicate signals of death and survival, further elucidating core immune functions.
Effective Research Tool
I recently used the Camelid Anti-PYCARD Recombinant Single Domain Antibody for my research on inflammation pathways, and found it to be a robust and reliable tool. The high sensitivity and specificity were especially beneficial for my experiments, enabling clear and reproducible results. It's a significant addition to my lab resources.
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For Research Use Only. Not For Clinical Use.
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