Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D3)
CAT#: VS7-0425-WR728
This product is a mouse IgG1 antibody that binds to human PYCARD. The antibody 1C3D3 recognizes specific amino acid domain of human PYCARD (AA: 1-120). It can be used for IHC, ICC, and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15948/36925_B_7_4_selected.jpg
Normal Tissue
Figure 3 Colon
Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_3.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (MCF7, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103490-PYCARD/subcellular
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Specifications
- Immunogen
- Recombinant human PYCARD (AA: 1-120) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PYCARD
- Species Reactivity
- Human
- Clone
- 1C3D3
- Molecule Class
- Adaptor Protein
- Applications
- IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Tumor suppressor
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:100 - 1:500
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- ASC; TMS; TMS1; CARD5; TMS-1
- Gene ID
- 29108
- UniProt ID
- Q9ULZ3
- Long Name
- PYD and CARD domain containing
- Cellular Localization
- Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Inflammasome; Membrane; Mitochondrion; Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_037390.2
- Function
- BMP receptor binding; cysteine-type endopeptidase activator activity; cysteine-type endopeptidase activator activity involved in apoptotic process; enzyme binding; identical protein binding; interleukin-6 receptor binding; myosin I binding; pattern recognition receptor activity; protease binding; protein dimerization activity; protein homodimerization activity; Pyrin domain binding; transmembrane transporter binding; tropomyosin binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PYCARD"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2948 | Hi-Affi™ Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (DS2948AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1506-CN | Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (CBACN-553) | WB, IF, FC | Rabbit IgG |
| PABS-0127 | Camelid Anti-PYCARD Recombinant Single Domain Antibody (Clone VHHASC) | ELISA, Block, FuncS | Camelid VHH |
| VS3-WK1213 | Rabbit Anti-PYCARD Recombinant Antibody (VS3-WK1213) | WB, ICC, IF, FC | Rabbit IgG |
| VS7-0425-WR729 | Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D7) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC807 | AbPlus™ Anti-PYCARD Magnetic Beads (VS-0724-YC807) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1834 | Anti-PYCARD Immunohistochemistry Kit | IHC | |
| VS-0525-XY5896 | Anti-Human PYCARD Immunohistochemistry Kit | IHC |
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