Mouse Anti-RAB1B Recombinant Antibody (clone 7A12G2)
CAT#: VS7-0425-WR736
This product is a mouse IgG2b antibody that binds to human RAB1B. The antibody 7A12G2 recognizes specific amino acid domain of human Rab1b (AA: 60-201). It can be used for IHC and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10225/931_C10_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10225/ihc_selected.jpg
Normal Tissue
Figure 3 Duodenum
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56141/143802_A_8_2.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56141/143802_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56141/143802_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174903-RAB1B
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Specifications
- Immunogen
- Recombinant human Rab1b (AA: 60-201) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human RAB1B
- Species Reactivity
- Human
- Clone
- 7A12G2
- Molecule Class
- Small GTPases
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAB1B, Member RAS Oncogene Family
- Gene ID
- 81876
- UniProt ID
- Q9H0U4
- Long Name
- RAB1B, Member RAS Oncogene Family
- Cellular Localization
- Cytoplasm; Membrane
- Post Translation Modifications
- Acetylation; Glycoprotein; Lipoprotein; Methylation; Phosphoprotein; Prenylation
- Protein Refseq
- NP_112243.1
- Function
- Hydrolase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "RAB1B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1741CT | Recombinant Mouse anti-Human RAB1B Monoclonal antibody (1B2) | ELISA, WB | |
| VS3-WK127 | Mouse Anti-RAB1B Recombinant Antibody (clone G2-A12) | WB, ELISA | Mouse IgG2b |
| VS3-WK1144 | Mouse Anti-RAB1B Recombinant Antibody (VS3-WK1144) | WB | Mouse IgG |
| VS3-FY2849 | Mouse Anti-RAB1B Recombinant Antibody (VS3-FY2849) | WB, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1846 | Anti-RAB1B Immunohistochemistry Kit | IHC |
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