Mouse Anti-RAB25 Recombinant Antibody (VS7-0425-WR738) (CAT#: VS7-0425-WR738)
This product is a mouse IgG antibody that binds to human RAB25. The antibody can be used for WB, IHC-P, IF, and ELISA assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
Figure 2 High expression in esophagus.
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, MCF7, OE19, RT4, SK-BR-3, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25
Specifications
- Immunogen
- Recombinant human RAB25 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RAB25
- Species Reactivity
- Human
- Molecule Class
- Small GTPases
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- PBS
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CATX-8; RAB11C
- Gene ID
- 57111
- UniProt ID
- P57735
- Long Name
- RAB25, member RAS oncogene family
- Cellular Localization
- Cell membrane; Cell projection; Cytoplasmic vesicle; Membrane
- Post Translation Modifications
- Lipoprotein; Methylation; Prenylation
- Protein Refseq
- NP_065120.2
- Function
- Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226).; Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity).; May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-044R | Mouse Anti-RAB25 Recombinant Antibody (ZG-044R) | WB, ELISA, IHC | Mouse IgG |
| VS3-XY1346 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| VS3-XY1347 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12F3) | ELISA, WB, IHC | Mouse IgG2a |
| VS13-YC967 | Rabbit Anti-RAB25 Recombinant Antibody (VS13-YC967) | WB | Rabbit IgG |
| VS7-0425-WR737 | Mouse Anti-RAB25 Monoclonal Antibody (VS7-0425-WR737) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
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