Rabbit Anti-RAB8A Recombinant Antibody (clone R06-8A7)
CAT#: VS3-FY1600
Clone R06-8A7 is a rabbit recombinant monoclonal antibody that exhibits specific detecting to human RAB8A. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. This Rab8A antibody was used in western blot analysis of IKK gamma in K562, Hela lysates and immunocytochemistry analysis of IKK gamma (green) in Hela using IKK gamma antibody.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/41945/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167461-RAB8A
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WB
Figure 1 Anti-RAB8A Antibody (VS3-FY1600) in WB.
The IKK gamma expression in K562, Hela lysates were detected using the anti-IKK gamma (RAB8A) by western blot assay.
ICC
Figure 2 Anti-RAB8A Antibody (VS3-FY1600) in ICC.
Immunocytochemistry staining of IKK gamma (green) in Hela using the anti-IKK gamma antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human RAB8A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RAB8A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-8A7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 24 kDa.
- Related Disease
- Microvillus Inclusion Disease and Open-Angle Glaucoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- RAB8A, Member RAS Oncogene Family; Mel Transforming Oncogene (Derived From Cell Line NK14); Oncogene C-Mel; RAB8; MEL; Mel Transforming Oncogene (Derived From Cell Line NK14)- RAB8 Homolog; Mel Transforming Oncogene (RAB8 Homolog); Ras-Associated Protein RAB8; Ras-Related Protein Rab-8A
- Gene ID
- 4218
- UniProt ID
- P61006
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle
- Post Translation Modifications
- Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
- Protein Refseq
- NP_005361.2
- Function
- The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
Regulates the compacted morphology of the Golgi.
Together with MYO5B and RAB11A participates in epithelial cell polarization.
Also involved in membrane trafficking to the cilium and ciliogenesis.
Together with MICALL2, may also regulate adherens junction assembly (By similarity).
May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RAB8A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-012CQ | Mouse Anti-Human Rab8 Antibody | ELISA, WB, FC | Mouse IgG |
| VS3-CJ1072 | Mouse Anti-RAB8A Recombinant Antibody (VS3-CJ1072) | WB | Mouse IgG1, κ |
| VS13-YC972 | Rabbit Anti-RAB8A Recombinant Antibody (VS13-YC972) | WB | Rabbit IgG |
| VS7-0425-WR747 | Mouse Anti-RAB8A Recombinant Antibody (clone 7G8B11) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5936 | Anti-RAB8A Immunohistochemistry Kit | IHC |
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