Mouse Anti-RAD50 Recombinant Antibody (clone 5A8E5)
CAT#: VS7-0425-WR758
This product is a mouse IgG1 antibody that binds to human RAD50. The antibody 5A8E5 recognizes specific amino acid domain of human RAD50 (AA: 228-359). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/ihc_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_9_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_9_5.jpg
Normal Tissue
Figure 7 Esophagus
Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_4_1.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113522-RAD50
❮
❯
Specifications
- Immunogen
- Recombinant human RAD50 (AA: 228-359) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RAD50
- Species Reactivity
- Human
- Clone
- 5A8E5
- Molecule Class
- Structural Maintenance Of Chromosomes (Smc) Protein
- Applications
- WB, ELSIA
- Conjugate
- Unconjugated
- Related Disease
- Nijmegen breakage syndrome-like disorder (NBSLD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; HRad50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); DNA Repair Protein RAD50; EC 3.6.3.27
- Gene ID
- 10111
- UniProt ID
- Q92878
- Long Name
- RAD50 Double Strand Break Repair Protein
- Cellular Localization
- Chromosome; Nucleus; Telomere
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_005723.2
- Function
- Hydrolase
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS7-0425-WR758. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "RAD50"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1431CT | Recombinant Mouse anti-Human RAD50 Monoclonal antibody (24C4/3D7) | ICC, IF, IHC-P, IP, WB | |
| VS3-WK107 | Mouse Anti-RAD50 Recombinant Antibody (clone G10-D2) | WB, ELISA | Mouse IgG2a |
| VS3-FY1241 | Recombinant Rabbit Anti-RAD50 Antibody (clone R07-8C0) | WB, IHC-P | Rabbit IgG |
| VS7-0425-WR759 | Mouse Anti-RAD50 Recombinant Antibody (clone 3D2G10) | WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2977 | Hi-Affi™ Recombinant Rabbit Anti-RAD50 Monoclonal Antibody (DS2977AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY431 | Mouse Anti-NHP RAD50 Recombinant Antibody (clone 13B3) | WB, IHC, IF | Mouse IgG1 |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: WB, ELISA, FuncS
Application: Neut, Inhib, FC, ELISA
Application: Block, IP, IF, FC
Application: ELISA, WB, FC, IHC, IP
Application: ELISA, FuncS
-2.png)
Application: ELISA
Application: ELISA, FC, FuncS
Application: ELISA, Vaccine, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
