Mouse Anti-RAD50 Recombinant Antibody (clone 5A8E5) (CAT#: VS7-0425-WR758)
This product is a mouse IgG1 antibody that binds to human RAD50. The antibody 5A8E5 recognizes specific amino acid domain of human RAD50 (AA: 228-359). It can be used for WB and ELISA assays.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/if_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/ihc_selected.jpg
Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/ihc_selected.jpg
(Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/ihc_selected.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_7_5.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_9_8.jpg
Figure 6 Cerebral cortex
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_9_5.jpg
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_9_5.jpg
Figure 7 Esophagus
(Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_4_1.jpg
(Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_4_1.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113522-RAD50
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113522-RAD50
Specifications
- Immunogen
- Recombinant human RAD50 (AA: 228-359) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RAD50
- Species Reactivity
- Human
- Clone
- 5A8E5
- Molecule Class
- Structural Maintenance Of Chromosomes (Smc) Protein
- Applications
- WB, ELSIA
- Conjugate
- Unconjugated
- Related Disease
- Nijmegen breakage syndrome-like disorder (NBSLD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; HRad50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); DNA Repair Protein RAD50; EC 3.6.3.27
- Gene ID
- 10111
- UniProt ID
- Q92878
- Long Name
- RAD50 Double Strand Break Repair Protein
- Cellular Localization
- Chromosome; Nucleus; Telomere
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_005723.2
- Function
- Hydrolase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "RAD50"
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Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1431CT | Recombinant Mouse anti-Human RAD50 Monoclonal antibody (24C4/3D7) | ICC/IF, IHC-P, IP, WB | |
| VS3-WK107 | Mouse Anti-RAD50 Recombinant Antibody (clone G10-D2) | WB, ELISA | Mouse IgG2a |
| VS3-FY1241 | Recombinant Rabbit Anti-RAD50 Antibody (clone R07-8C0) | WB, IHC-P | Rabbit IgG |
| VS7-0425-WR759 | Mouse Anti-RAD50 Recombinant Antibody (clone 3D2G10) | WB, IHC, FC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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