Mouse Anti-NHP RAD50 Recombinant Antibody (clone 13B3) (CAT#: VS-1024-XY431)
Designed for the detection of RAD50, this mouse monoclonal antibody exhibits reactivity with human, mouse, non-human primate, and rat samples. The immunogen consists of amino acids 1-425 of RAD50, ensuring targeted specificity. Suitable for use in Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this antibody is a crucial reagent for investigating RAD50 expression and its role in various biological processes, providing reliable performance across multiple experimental platforms.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/if_selected.jpg

(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/ihc_selected.jpg

(Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/ihc_selected.jpg

(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_7_5.jpg

(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_9_8.jpg

(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_9_5.jpg

(Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_4_1.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113522-RAD50
Specifications
- Immunogen
- Amino acids 1-425 of RAD50
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- RAD50
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 13B3
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:3,000, IHC: 1:100-1:800, IF: 1:100-1:800
Target
- Alternative Names
- RAD50 Double Strand Break Repair Protein; RAD50 Homolog; Double Strand Break Repair Protein; HRad50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); DNA Repair Protein RAD50; EC 3.6.3.27
- Gene ID
- 10111
- UniProt ID
- Q92878
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RAD50"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1431CT | Recombinant Mouse anti-Human RAD50 Monoclonal antibody (24C4/3D7) | ICC/IF, IHC-P, IP, WB | |
VS3-WK107 | Mouse Anti-RAD50 Recombinant Antibody (clone G10-D2) | WB, ELISA | Mouse IgG2a |
VS3-FY1241 | Recombinant Rabbit Anti-RAD50 Antibody (clone R07-8C0) | WB, IHC-P | Rabbit IgG |
VS7-0425-WR758 | Mouse Anti-RAD50 Recombinant Antibody (clone 5A8E5) | WB, ELSIA | Mouse IgG1 |
VS7-0425-WR759 | Mouse Anti-RAD50 Recombinant Antibody (clone 3D2G10) | WB, IHC, FC | Mouse IgG2a |
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