Mouse Anti-NHP RAD50 Recombinant Antibody (clone 13B3)
CAT#: VS-1024-XY431
Designed for the detection of RAD50, this mouse monoclonal antibody exhibits reactivity with human, mouse, non-human primate, and rat samples. The immunogen consists of amino acids 1-425 of RAD50, ensuring targeted specificity. Suitable for use in Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this antibody is a crucial reagent for investigating RAD50 expression and its role in various biological processes, providing reliable performance across multiple experimental platforms.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52291/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/ihc_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_A_9_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_9_5.jpg
Normal Tissue
Figure 7 Esophagus
Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22103/49527_B_4_1.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113522-RAD50
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Specifications
- Immunogen
- Amino acids 1-425 of RAD50
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- RAD50
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 13B3
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:3,000, IHC: 1:100-1:800, IF: 1:100-1:800
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RAD50"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1431CT | Recombinant Mouse anti-Human RAD50 Monoclonal antibody (24C4/3D7) | ICC, IF, IHC-P, IP, WB | |
| VS3-WK107 | Mouse Anti-RAD50 Recombinant Antibody (clone G10-D2) | WB, ELISA | Mouse IgG2a |
| VS3-FY1241 | Recombinant Rabbit Anti-RAD50 Antibody (clone R07-8C0) | WB, IHC-P | Rabbit IgG |
| VS7-0425-WR758 | Mouse Anti-RAD50 Recombinant Antibody (clone 5A8E5) | WB, ELSIA | Mouse IgG1 |
| VS7-0425-WR759 | Mouse Anti-RAD50 Recombinant Antibody (clone 3D2G10) | WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2977 | Hi-Affi™ Recombinant Rabbit Anti-RAD50 Monoclonal Antibody (DS2977AB) | WB | IgG |
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