Recombinant Rabbit Anti-RAD51 Monoclonal Antibody (JM54-26)
CAT#: MRO-1314-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to RAD51.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/493_D2_3_selected.jpg
Normal Tissue
Figure 2 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Low Intensity: Weak Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/91890_A_6_6.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/91890_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39310/91890_A_7_3.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000051180-RAD51
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Specifications
- Immunogen
- Recombinant protein within human Rad51 aa 1-150
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human
- Species Reactivity
- Human, Mouse, Rat
- Clone
- JM54-26
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
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Superior Nuclear Foci Staining
We used the Recombinant Rabbit Anti-RAD51 Antibody (Clone JM54-26) to visualize DNA repair foci in irradiated cells. The immunofluorescence images were incredibly crisp, showing clear nuclear foci with minimal background. This monoclonal antibody is far superior to the polyclonal sera we previously used for this application.
Reliable for DNA Repair
This antibody has been a workhorse in our DNA damage response research. It works consistently in Western Blotting, IP, and IHC. We rely on it to monitor homologous recombination repair efficiency in our experimental models. The recombinant production guarantees reproducibility.
Q&As
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Is this antibody suitable for detecting DNA repair foci by IF?
A: Yes, this Recombinant Rabbit Anti-RAD51 Antibody (Clone JM54-26) is validated for Immunofluorescence (IF), making it highly suitable for visualizing RAD51 foci in the nucleus of cells.
-
Does this antibody work on rat samples?
A: Yes, this antibody reacts with RAD51 from Human, Mouse, and Rat, providing flexibility for DNA repair research across different mammalian models.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone JM54-26"
- CAT
- Product Name
See other products for "RAD51"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3172z | Mouse Anti-RAD51 Recombinant Antibody (clone 12G11) | WB, ICC, IF, IHC, IP | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1432CT | Recombinant Mouse anti-Human RAD51 Monoclonal antibody (24F5) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2978 | Hi-Affi™ Recombinant Rabbit Anti-RAD51 Monoclonal Antibody (DS2978AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1240CQ | Mouse Anti-RAD51 Recombinant Antibody (clone 3C10) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-051R | Mouse Anti-Rad51 Recombinant Antibody (ZG-051R) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX952 | Mouse Anti-RAD51 Recombinant Antibody (clone 1281CT886.273.179.159) | WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1135 | AbPlus™ Anti-RAD51 Magnetic Beads (VS-0724-YC1135) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC978 | CytoStream™ Rabbit Anti-RAD51 Recombinant Antibody (VS13-YC978) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1864 | Anti-RAD51 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5957 | Anti-Mouse RAD51 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5956 | Anti-Human RAD51 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5958 | Anti-Rat RAD51 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC77 | Anti-RAD51 Antibody Prodrug, Protease Activated (3C10) | ISZ, Cyt, FuncS |
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