Mouse Anti-RAN Recombinant Antibody (clone 8D1H12)
CAT#: VS7-0425-WR765
This product is a mouse IgG1 antibody that binds to human RAN. The antibody 8D1H12 recognizes specific amino acid domain of human RAN (AA: 1-216). It can be used for WB, IHC, and FC assays. It cross reacts with mouse and monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63353/1392_C11_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132341-RAN
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Specifications
- Immunogen
- Recombinant human RAN (AA: 1-216) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RAN
- Species Reactivity
- Human, Monkey, Rat
- Clone
- 8D1H12
- Molecule Class
- Small GTPases
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAN; GTPase Ran; Androgen Receptor-Associated Protein 24; Gsp1; Guanosine Triphosphatase Ran; OK/SW-Cl.81; Member RAS Oncogene Family; TC4; GTP-Binding Nuclear Protein Ran
- Gene ID
- 5901
- UniProt ID
- P62826
- Long Name
- RAN, Member RAS Oncogene Family
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001287725.1
- Function
- Hydrolase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "RAN"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2400z | Mouse Anti-RAN Recombinant Antibody (clone 26D12) | WB, ELISA, ICC, IF, IP | Mouse IgG2b |
| VS3-QX956 | Mouse Anti-RAN Recombinant Antibody (clone A1-6D) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS3-FY1868 | Rabbit Anti-RAN Recombinant Antibody (clone R01-7J8) | WB, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2992 | Hi-Affi™ Recombinant Rabbit Anti-Ran Monoclonal Antibody (DS2992AB) | WB, ICC, IF, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY438 | Mouse Anti-NHP RAN Recombinant Antibody (clone 8D1A6) | WB, IHC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1871 | Anti-RAN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5975 | Anti-Rat RAN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5974 | Anti-Human RAN Immunohistochemistry Kit | IHC |
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