Rabbit Anti-RAN Recombinant Antibody (clone R01-7J8) (CAT#: VS3-FY1868)
Clone R01-7J8 is a rabbit recombinant monoclonal antibody that exhibits specific detecting to human RAN. The RAN protein has a calculated weight of 24 kDa. It cross-reacts with mouse, monkey.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R01-7J8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200), immunoprecipitation (dilution range 1:20).
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63353/1392_C11_2_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63353/1392_C11_2_selected.jpg
Figure 2 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132341-RAN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132341-RAN
Specifications
- Immunogen
- Synthetic peptide of human Ran
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Monkey RAN
- Species Reactivity
- Human, Mouse, Monkey
- Clone
- R01-7J8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 24 kDa.
- Related Disease
- Kennedy's disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- RAN, Member RAS Oncogene Family; Androgen Receptor-Associated Protein 24; Ras-Related Nuclear Protein; Ras-Like Protein TC4; GTPase Ran; ARA24; GTP-Binding Nuclear Protein Ran
- Gene ID
- 5901
- UniProt ID
- P62826
- Sequence Similarities
- Belongs to the small GTPase superfamily. Ran family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Acetylation by KAT5 at Lys-134 is increased during mitosis, impairs RANGRF binding and enhances RCC1 binding (PubMed:29040603).
Acetylation at Lys-37 enhances the association with nuclear export components (PubMed:31075303).
Deacetylation of Lys-37 by SIRT7 regulates the nuclear export of NF-kappa-B subunit RELA/p65 (PubMed:31075303).
- Protein Refseq
- NP_001287725.1; NP_001287726.1; NP_006316.1
- Function
- Acts as a negative regulator of the kinase activity of VRK1 and VRK2.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2400z | Mouse Anti-RAN Recombinant Antibody (clone 26D12) | WB, ELISA, ICC, IF, IP | Mouse IgG2b |
| VS3-QX956 | Mouse Anti-RAN Recombinant Antibody (clone A1-6D) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR765 | Mouse Anti-RAN Recombinant Antibody (clone 8D1H12) | WB, IHC, FC | Mouse IgG1 |
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