Rabbit Anti-RB1 Recombinant Antibody (VS3-FY2861)
CAT#: VS3-FY2861
This anti-RB1 antibody is a rabbit recombinant monoclonal antibody that was designed to bind the human RB1 protein. It cross-reacts with rat species. The monoclonal antibody can be used in the area of Retinoblastoma and Small Cell Cancer Of The Lung. This monoclonal antibody is suitable for employment in Immunoprecipitation (1/20), Western Blot (1/500-1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/95/656_B4_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139687-RB1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat RB1
- Species Reactivity
- Human, Rat
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Retinoblastoma and Small Cell Cancer Of The Lung
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Target
- Alternative Names
- Retinoblastoma; Rb
- Gene ID
- 5925
- UniProt ID
- A8K5E3
- Sequence Similarities
- Belongs to the retinoblastoma protein (RB) family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys265, Lys279, Lys341, Lys810, Lys847, and Lys900 (NX_P06400 [NX_P06400-1])
Modification sites at PhosphoSitePlus (P06400)
Glycosylation from GlyGen (P06400) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001394094.1; NP_001394095.1; NP_001394096.1; NP_001394097.1
- Function
- Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802).
The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802).
Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802).
Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase.
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3015 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (DS3015AB) | ICC, IF, IP, WB | IgG |
| MOR-4704 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH218DS) | WB, IF, ICC, FC | IgG |
| MOR-4705 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH219DS) | WB, IF, ICC, FC | IgG |
| MOR-4706 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH220DS) | WB, IF, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-060R | Mouse Anti-RB1 Recombinant Antibody (ZG-060R) | ELISA, IHC | Mouse IgG |
| VS3-XY1360 | Mouse Anti-RB1 Recombinant Antibody (clone 7E4B8) | ELISA, IHC | Mouse IgG1 |
| VS3-XY1361 | Mouse Anti-RB1 Recombinant Antibody (clone 2A11D11) | ICC, FC | Mouse IgG1 |
| VS3-XY1362 | Mouse Anti-RB1 Recombinant Antibody (clone 2A11D4) | FC | Mouse IgG1 |
| VS3-WK2264 | Rabbit Anti-RB1 (pS811) Recombinant Antibody (VS3-WK2264) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6011 | Anti-RB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6012 | Anti-Mouse RB1 Immunohistochemistry Kit | IHC |
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