Rabbit Anti-RB1 Recombinant Antibody (VS3-FY2861) (CAT#: VS3-FY2861)
This anti-RB1 antibody is a rabbit recombinant monoclonal antibody that was designed to bind the human RB1 protein. It cross-reacts with rat species. The monoclonal antibody can be used in the area of Retinoblastoma and Small Cell Cancer Of The Lung. This monoclonal antibody is suitable for employment in Immunoprecipitation (1/20), Western Blot (1/500-1/1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/95/656_B4_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/95/656_B4_1_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_5.jpg
Figure 6 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139687-RB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139687-RB1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat RB1
- Species Reactivity
- Human, Rat
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Retinoblastoma and Small Cell Cancer Of The Lung
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Target
- Alternative Names
- Retinoblastoma; Rb
- Gene ID
- 5925
- UniProt ID
- A8K5E3
- Sequence Similarities
- Belongs to the retinoblastoma protein (RB) family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys265, Lys279, Lys341, Lys810, Lys847, and Lys900 (NX_P06400 [NX_P06400-1])
Modification sites at PhosphoSitePlus (P06400)
Glycosylation from GlyGen (P06400) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001394094.1; NP_001394095.1; NP_001394096.1; NP_001394097.1
- Function
- Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802).
The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802).
Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802).
Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RB1"
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3015 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (DS3015AB) | ICC/IF, IP, WB | IgG |
| MOR-4704 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH218DS) | WB, IF, ICC, FC | IgG |
| MOR-4705 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH219DS) | WB, IF, ICC, FC | IgG |
| MOR-4706 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH220DS) | WB, IF, ICC | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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